Golden bananas in the field: elevated fruit pro-vitamin A from the expression of a single banana transgene.
Jean-Yves Paul,Harjeet Khanna,Jennifer Kleidon,Phuong Hoang,Jason Geijskes,Jeffrey Daniells,Ella Zaplin,Yvonne Rosenberg,Anthony P. James,Bulukani Mlalazi,Pradeep C. Deo,Geofrey Arinaitwe,Priver Namanya,Priver Namanya,Douglas K. Becker,Jimmy Moses Tindamanyire,Wilberforce Tushemereirwe,Robert M. Harding,James L. Dale +18 more
Reads0
Chats0
TLDR
The results from this study suggest that early activation of the rate‐limiting enzyme in the carotenoid biosynthetic pathway and extended fruit maturation time are essential factors to achieve optimal PVA concentrations in banana fruit.Abstract:
Summary Vitamin A deficiency remains one of the world's major public health problems despite food fortification and supplements strategies. Biofortification of staple crops with enhanced levels of pro-vitamin A (PVA) offers a sustainable alternative strategy to both food fortification and supplementation. As a proof of concept, PVA-biofortified transgenic Cavendish bananas were generated and field trialed in Australia with the aim of achieving a target level of 20 μg/g of dry weight (dw) β-carotene equivalent (β-CE) in the fruit. Expression of a Fe'i banana-derived phytoene synthase 2a (MtPsy2a) gene resulted in the generation of lines with PVA levels exceeding the target level with one line reaching 55 μg/g dw β-CE. Expression of the maize phytoene synthase 1 (ZmPsy1) gene, used to develop “Golden Rice 2”, also resulted in increased fruit PVA levels although many lines displayed undesirable phenotypes. Constitutive expression of either transgene with the maize polyubiquitin promoter increased PVA accumulation from the earliest stage of fruit development. In contrast, PVA accumulation was restricted to the late stages of fruit development when either the banana 1-aminocyclopropane-1-carboxylate oxidase or the expansin 1 promoters were used to drive the same transgenes. Wild-type plants with the longest fruit development time had also the highest fruit PVA concentrations. The results from this study suggest that early activation of the rate-limiting enzyme in the carotenoid biosynthetic pathway, as well as extended fruit maturation time, are essential factors to achieve optimal PVA concentrations in banana fruit.read more
Citations
More filters
Journal ArticleDOI
Genetic strategies for improving crop yields
Julia Bailey-Serres,Julia Bailey-Serres,Jane E. Parker,Elizabeth A. Ainsworth,Giles E. D. Oldroyd,Julian I. Schroeder +5 more
TL;DR: The potential of plant sciences to address post-Green Revolution challenges in agriculture is considered and emerging strategies for enhancing sustainable crop production and resilience in a changing climate are explored.
Journal ArticleDOI
Carotenoid Metabolism in Plants: The Role of Plastids.
TL;DR: This review provides a comprehensive overview of the impact of various types of plastids on carotenoid biosynthesis and accumulation, and discusses recent advances in the understanding of the regulatory control ofCarotenogenesis and metabolic engineering ofcarotenoids in light ofplastid types in plants.
Journal ArticleDOI
Provitamin A biofortification of crop plants: a gold rush with many miners.
TL;DR: This review presents the state of the art and the areas that need further experimentation in biofortification of plant-derived foods with provitamin A carotenoids, using transgenic approaches as well as conventional breeding.
Journal ArticleDOI
A comprehensive investigation of starch degradation process and identification of a transcriptional activator MabHLH6 during banana fruit ripening.
TL;DR: Findings suggest that starch degradation during banana fruit ripening may be attributed to the complex actions of numerous enzymes related to starch breakdown at transcriptional and translational levels, and that MabHLH6 may act as a positive regulator of this process via direct activation of a series of starch degradation‐related genes.
Journal ArticleDOI
CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for β-carotene biosynthesis in banana fruit.
Navneet Kaur,Anshu Alok,Shivani,Pankaj Kumar,Navjot Kaur,Praveen Awasthi,Siddhant Chaturvedi,Pankaj Pandey,Ashutosh Pandey,Ajay Kumar Pandey,Siddharth Tiwari +10 more
TL;DR: This is the first report in banana to improve nutritional trait by using a precise genome editing approach using the CRISPR/Cas9 approach.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book
Molecular cloning : a laboratory manual
Joseph Sambrook,David W. Russell +1 more
TL;DR: The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Journal ArticleDOI
Analyzing real-time PCR data by the comparative C(T) method.
TL;DR: This protocol provides an overview of the comparative CT method for quantitative gene expression studies and various examples to present quantitative gene Expression data using this method.
Journal ArticleDOI
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
TL;DR: GUS is very stable, and tissue extracts continue to show high levels of GUS activity after prolonged storage, and Histochemical analysis has been used to demonstrate the localization of gene activity in cells and tissues of transformed plants.
Related Papers (5)
Engineering the Provitamin A (β-Carotene) Biosynthetic Pathway into (Carotenoid-Free) Rice Endosperm
The banana (Musa acuminata) genome and the evolution of monocotyledonous plants
Angélique D'Hont,Jean-Marc Aury,Franc-Christophe Baurens,Françoise Carreel,Olivier Garsmeur,Benjamin Noel,Stéphanie Bocs,Gaëtan Droc,Mathieu Rouard,Corinne Da Silva,Kamel Jabbari,Kamel Jabbari,Kamel Jabbari,Céline Cardi,Julie Poulain,Marlã̈ne Souquet,Karine Labadie,Cyril Jourda,Juliette Lengellé,Marguerite Rodier-Goud,Adriana Alberti,Maria Bernard,Margot Correa,Saravanaraj Ayyampalayam,Michael R. McKain,Jim Leebens-Mack,Diane Burgess,Michael Freeling,Didier Mbéguié-A-Mbéguié,Matthieu Chabannes,Thomas Wicker,Olivier Panaud,Jose Barbosa,E. Hribova,Pat Heslop-Harrison,Rémy Habas,Ronan Rivallan,Philippe Francois,Claire Poiron,Andrzej Kilian,Dheema Burthia,Christophe Jenny,Frédéric Bakry,Spencer Brown,Valentin Guignon,Valentin Guignon,Gert H. J. Kema,Miguel A. Dita,Cees Waalwijk,Steeve Joseph,Anne Dievart,Olivier Jaillon,Olivier Jaillon,Olivier Jaillon,Julie Leclercq,Xavier Argout,Eric Lyons,Ana Maria Rocha de Almeida,Mouna Jeridi,Jaroslav Dolezel,Nicolas Roux,Ange-Marie Risterucci,Jean Weissenbach,Jean Weissenbach,Jean Weissenbach,Manuel Ruiz,Jean-Christophe Glaszmann,Francis Quetier,Nabila Yahiaoui,Patrick Wincker,Patrick Wincker,Patrick Wincker +71 more