Journal ArticleDOI
Human TLR3 recognizes dengue virus and modulates viral replication in vitro
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TLDR
It is found that the expression of TLR3 can mediate strong IFN‐α/β release and inhibit DENV viral replication significantly, thus limit the cytopathic effect.Abstract:
The elicitation of large amount inflammatory cytokine in serum has been developed as the cause of the plasma leakage in dengue fever (DF)/dengue haemorrhagic fever (DHF) infection. Virus recognition in innate immunity is the key. The Toll-like receptors (TLRs) play an important role in pathogen recognition towards cytokine induction among several viruses; however, the role of TLRs on innate immune recognition against DENV remains unclear. This study aims at the interaction between dengue virus (DENV) and human TLRs at the incipient stage of infection in vitro. Our experiment reveals that stably expression of TLR3, 7, 8 on HEK293 enables IL-8 secretion after DENV recognition. By the model of human monocytic cells U937, we demonstrated the trigger of IL-8 after viral recognition of human monocytic cell is primary through TLR3 following endosomal acidification. Silencing of TLR3 in U937 cells significantly blocks the DENV-induced IL-8 production. Besides, the interaction is further corroborated by colocalization of TLR3 and DENV RNA upon DENV internalization. Furthermore, in this study we found the expression of TLR3 can mediate strong IFN-alpha/beta release and inhibit DENV viral replication significantly, thus limit the cytopathic effect.read more
Citations
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Journal ArticleDOI
Zika Virus Depletes Neural Progenitors in Human Cerebral Organoids through Activation of the Innate Immune Receptor TLR3.
Jason Dang,Shashi Kant Tiwari,Gianluigi Lichinchi,Yue Qin,Veena S. Patil,Alexey Eroshkin,Tariq M. Rana +6 more
TL;DR: It is shown that a prototype strain of ZIKV, MR766, efficiently infects organoids and causes a decrease in overall organoid size that correlates with the kinetics of viral copy number.
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Toll-like receptors in antiviral innate immunity
Sandra N. Lester,Kui Li +1 more
TL;DR: The current knowledge of the roles TLRs play in antiviral innate immune responses is reviewed, examples of TLR-mediated viral recognition are discussed, and strategies used by viruses to antagonize the host antiviral inherent immune responses are described.
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Dengue virus life cycle: viral and host factors modulating infectivity
TL;DR: An overview of the infectious life cycle of DENV is given and the viral and host factors that are important in controlling DENV infection are discussed.
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DENV inhibits type I IFN production in infected cells by cleaving human STING.
Sebastian Aguirre,Ana M. Maestre,Sarah Pagni,Jenish R. Patel,Timothy Savage,Delia Gutman,Kevin Maringer,Dabeiba Bernal-Rubio,Reed S. Shabman,Viviana Simon,Juan R. Rodriguez-Madoz,Juan R. Rodriguez-Madoz,Lubbertus C. F. Mulder,Glen N. Barber,Ana Fernandez-Sesma +14 more
TL;DR: It is shown that DENV NS2B3 is not able to degrade the mouse version of STING, a phenomenon that severely restricts the replication of DENV in mouse cells, suggesting that STING plays a key role in the inhibition ofDENV infection and spread in mice.
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RIG-I, MDA5 and TLR3 synergistically play an important role in restriction of dengue virus infection.
TL;DR: It is demonstrated that the intracellular RNA virus sensors (RIG-I, MDA5 and TLR3) are activated upon DV infection and are essential for host defense against the virus.
References
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Journal ArticleDOI
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TL;DR: Recognition of microbial infection and initiation of host defense responses is controlled by multiple mechanisms and recent studies have provided important clues about the mechanisms of TLR-mediated control of adaptive immunity orchestrated by dendritic cell populations in distinct anatomical locations.
Journal ArticleDOI
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