Integrated, Step-Wise, Mass-Isotopomeric Flux Analysis of the TCA Cycle
Tiago C. Alves,Rebecca L. Pongratz,Xiaojian Zhao,Orlando Yarborough,Sam Sereda,Orian S. Shirihai,Gary W. Cline,Graeme F. Mason,Richard G. Kibbey +8 more
TLDR
MIMOSA measures key metabolic rates from the precursor/product position-specific transfer of (13)C-label between metabolites and has broad applicability to any glucose-oxidizing cell.About:
This article is published in Cell Metabolism.The article was published on 2015-11-03 and is currently open access. It has received 91 citations till now. The article focuses on the topics: Pyruvate decarboxylation & Pyruvate carboxylase.read more
Citations
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Metabolomics and Metabolic Diseases: Where Do We Stand?
TL;DR: Progress in metabolomics and challenges for the future are reviewed here.
Journal ArticleDOI
Metabolic Flexibility as an Adaptation to Energy Resources and Requirements in Health and Disease.
TL;DR: The breadth and depth of metabolic flexibility and its impact on health and disease are discussed and important advances in metabolic flexibility research are outlined and medical horizons and translational aspects are outlined.
Journal ArticleDOI
Investigating mitochondrial redox state using NADH and NADPH autofluorescence
TL;DR: Recent studies using fluorescence lifetime imaging microscopy (FLIM) which offer the potential to discriminate between the two separate pools of NADH and NADPH are discussed, offering biochemical insights into the changes in time-resolved NAD(P)H fluorescence signals observed in diseased tissues.
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Metabolomics: A Primer
Xiaojing Liu,Jason W. Locasale +1 more
TL;DR: This primer provides an overview of metabolomics, especially mass spectrometry (MS-based metabolomics), which uses liquid chromatography (LC) for separation, and discusses its utilities and limitations.
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FGF-dependent metabolic control of vascular development
Pengchun Yu,Kerstin Wilhelm,Alexandre Dubrac,Joe K. Tung,Tiago C. Alves,Jennifer S. Fang,Yi Xie,Jie Zhu,Zehua Chen,Frederik De Smet,Frederik De Smet,Jiasheng Zhang,Suk-Won Jin,Suk-Won Jin,Lele Sun,Hongye Sun,Richard G. Kibbey,Karen K. Hirschi,Nissim Hay,Peter Carmeliet,Thomas Chittenden,Anne Eichmann,Michael Potente,Michael Simons +23 more
TL;DR: FGF-dependent regulation of endothelial glycolysis is a pivotal process in developmental and adult vascular growth and development and FGF receptor (FGFR) signalling is identified as a critical regulator of vascular development.
References
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A roadmap for interpreting 13 C metabolite labeling patterns from cells
Joerg Martin Buescher,Maciek R. Antoniewicz,Laszlo G. Boros,Shawn C. Burgess,Henri Brunengraber,Clary B. Clish,Ralph J. DeBerardinis,Olivier Feron,Christian Frezza,Bart Ghesquière,Eyal Gottlieb,Karsten Hiller,Russell G. Jones,Jurre J. Kamphorst,Richard G. Kibbey,Alec C. Kimmelman,Jason W. Locasale,Sophia Y. Lunt,Oliver D. K. Maddocks,Craig R. Malloy,Christian M. Metallo,Emmanuelle J. Meuillet,Joshua Munger,Katharina Nöh,Joshua D. Rabinowitz,Markus Ralser,Markus Ralser,Uwe Sauer,Gregory Stephanopoulos,Julie St-Pierre,Daniel A. Tennant,Christoph Wittmann,Matthew G. Vander Heiden,Matthew G. Vander Heiden,Alexei Vazquez,Karen H. Vousden,Jamey D. Young,Nicola Zamboni,Sarah-Maria Fendt +38 more
TL;DR: Key issues in interpreting (13)C metabolite labeling patterns are reviewed, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.
A roadmap for interpreting 13C metabolite labeling patterns from cells
TL;DR: In this paper, the authors review key issues in interpreting (13)C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.
Journal ArticleDOI
INCA: a computational platform for isotopically non-stationary metabolic flux analysis.
TL;DR: Isotopomer network compartmental analysis (INCA) is the first publicly available software package that can perform both steady-state metabolic flux analysis and isotopically non-stationary metabolic fluxAnalysis.
Journal ArticleDOI
Evaluation of carbon flux and substrate selection through alternate pathways involving the citric acid cycle of the heart by 13C NMR spectroscopy.
TL;DR: Equations are presented which allow this same analysis on multiply labeled substrates, making this technique extremely powerful for the evaluation of substrate selection and relative metabolic flux through anaplerotic and oxidative pathways in the intact heart.
Journal ArticleDOI
Mass isotopomer distribution analysis: a technique for measuring biosynthesis and turnover of polymers.
TL;DR: MIDA allows dynamic measurements to be made through analyses on a polymer alone during both isotopic incorporation and decay phases, and is potentially applicable to nucleic acids, porphyrins, perhaps proteins, and many other classes of polymers.
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