Citations
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Crystallization and cryocrystallography inside X-ray capillaries
TL;DR: In this article, a modification of the gel acupuncture method is presented to grow isolated crystals inside X-ray capillaries without any post-crystallization manipulation, and two additional elements are proposed for inclusion in the cryosystems currently in use: a laser pointer to illuminate the crystal to be flash-cooled and a trap to divert the N2 flow and switch from room temperature to 100
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SYMMODES: a software package for group-theoretical analysis of structural phase transitions
TL;DR: In this paper, the authors present a tool for a group-theoretical analysis of a structural phase transition, including a detailed group±subgroup description of the chain G > H and a calculation of the primary and secondary modes for the symmetry break G! H.
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Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus.
TL;DR: The structure of Pfu elucidates the electron density of the interface between the exonuclease and thumb domains, which has not been previously observed in the KOD1 structure, and shows that both the Y-GG/A and beta-hairpin motifs of P Fu are found to differ with that of K OD1, and may explain differences in processivity.
Journal ArticleDOI
Progress in rational methods of cryoprotection in macromolecular crystallography
Thomas Alcorn,Douglas H. Juers +1 more
TL;DR: Measurements of the average thermal contractions of 26 different cryosolutions are presented and discussed in conjunction with other recent advances in the rational design of protocols for cryogenic cooling in macromolecular crystallography.
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NIST Standard Reference Material 3600: Absolute Intensity Calibration Standard for Small-Angle X-ray Scattering
TL;DR: The certification of a new NIST standard reference material for the calibration of small-angle X-ray scattering intensity is described, including the purpose, use and applicability of the SRM together with limitations and uncertainties in the intensity calibration provided.
References
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Journal ArticleDOI
The Protein Data Bank
Helen M. Berman,John D. Westbrook,Zukang Feng,Gary L. Gilliland,Talapady N. Bhat,Helge Weissig,Ilya N. Shindyalov,Philip E. Bourne +7 more
TL;DR: The goals of the PDB are described, the systems in place for data deposition and access, how to obtain further information and plans for the future development of the resource are described.
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Crystal structure refinement with SHELXL
TL;DR: New features added to the refinement program SHELXL since 2008 are described and explained.
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Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
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PHENIX: a comprehensive Python-based system for macromolecular structure solution
Paul D. Adams,Paul D. Adams,Pavel V. Afonine,Gábor Bunkóczi,Vincent B. Chen,Ian W. Davis,Nathaniel Echols,Jeffrey J. Headd,Li-Wei Hung,Gary J. Kapral,Ralf W. Grosse-Kunstleve,Airlie J. McCoy,Nigel W. Moriarty,Robert D. Oeffner,Randy J. Read,David S. Richardson,Jane S. Richardson,Thomas C. Terwilliger,Peter H. Zwart +18 more
TL;DR: The PHENIX software for macromolecular structure determination is described and its uses and benefits are described.
Journal ArticleDOI
Phaser crystallographic software
Airlie J. McCoy,Ralf W. Grosse-Kunstleve,Paul D. Adams,Martyn Winn,Laurent C. Storoni,Randy J. Read +5 more
TL;DR: A description is given of Phaser-2.1: software for phasing macromolecular crystal structures by molecular replacement and single-wavelength anomalous dispersion phasing.