Joint profiling of histone modifications and transcriptome in single cells from mouse brain.
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TLDR
Paired-Tag as discussed by the authors was used to profile five histone modifications jointly with transcriptome in the adult mouse frontal cortex and hippocampus to produce cell-type-resolved maps of chromatin state and transcriptome.Abstract:
Genome-wide profiling of histone modifications can reveal not only the location and activity state of regulatory elements, but also the regulatory mechanisms involved in cell-type-specific gene expression during development and disease pathology. Conventional assays to profile histone modifications in bulk tissues lack single-cell resolution. Here we describe an ultra-high-throughput method, Paired-Tag, for joint profiling of histone modifications and transcriptome in single cells to produce cell-type-resolved maps of chromatin state and transcriptome in complex tissues. We used this method to profile five histone modifications jointly with transcriptome in the adult mouse frontal cortex and hippocampus. Integrative analysis of the resulting maps identified distinct groups of genes subject to divergent epigenetic regulatory mechanisms. Our single-cell multiomics approach enables comprehensive analysis of chromatin state and gene regulation in complex tissues and characterization of gene regulatory programs in the constituent cell types.read more
Citations
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Single-cell nuclear architecture across cell types in the mouse brain.
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TL;DR: In this article, the authors correlate multimodal information across thousands of single cells in mouse tissues, including gene expression programs, chromatin states, and nuclear architectures, to correlate such multi-modal information.
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Dictionary learning for integrative, multimodal, and scalable single-cell analysis
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TL;DR: ‘bridge integration’ is introduced, a method to harmonize singlecell datasets across modalities by leveraging a multi-omic dataset as a molecular bridge, which aims to broaden the utility of single-cell reference datasets and facilitate comparisons across diverse molecular modalities.
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