Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii.
Daniel E. Voth,Robert A. Heinzen +1 more
Reads0
Chats0
TLDR
Current understanding of the cellular events that occur during parasitism of host cells by Coxiella, including deployment of a type IV secretion system to deliver effector proteins to the host cytosol is summarized.Abstract:
Summary
Most intracellular parasites employ sophisticated mechanisms to direct biogenesis of a vacuolar replicative niche that circumvents default maturation through the endolysosomal cascade. However, this is not the case of the Q fever bacterium, Coxiella burnetii. This hardy, obligate intracellular pathogen has evolved to not only survive, but to thrive, in the harshest of intracellular compartments: the phagolysosome. Following internalization, the nascent Coxiella phagosome ultimately develops into a large and spacious parasitophorous vacuole (PV) that acquires lysosomal characteristics such as acidic pH, acid hydrolases and cationic peptides, defences designed to rid the host of intruders. However, transit of Coxiella to this environment is initially stalled, a process that is apparently modulated by interactions with the autophagic pathway. Coxiella actively participates in biogenesis of its PV by synthesizing proteins that mediate phagosome stalling, autophagic interactions, and development and maintenance of the mature vacuole. Among the potential mechanisms mediating these processes is deployment of a type IV secretion system to deliver effector proteins to the host cytosol. Here we summarize our current understanding of the cellular events that occur during parasitism of host cells by Coxiella.read more
Citations
More filters
Journal ArticleDOI
Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.
TL;DR: In this article, the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI) were examined.
Journal ArticleDOI
Involvement of plasma membrane H+-ATPase in the ammonium-nutrition response of barley roots
Maoxing Zhang,Ming Ding,Feiyun Xu,Muhammad Rahil Afzal,Xi Chen,Houqing Zeng,Feng Yan,Yiyong Zhu +7 more
Journal ArticleDOI
Distribution of Eg5 and TPX2 in mitosis: Insight from CRISPR tagged cells.
TL;DR: The results show that using cells with fluorescent tags at the endogenous locus can provide novel insight into protein distribution during mitosis and tag Eg5 and TPX2 with EGFP and quantify protein distribution throughout mitosis.
Journal ArticleDOI
Mechanisms of action of Coxiella burnetii effectors inferred from host-pathogen protein interactions.
Anders Wallqvist,Hao Wang,Nela Zavaljevski,Vesna Memišević,Keehwan Kwon,Rembert Pieper,Seesandra V. Rajagopala,Jaques Reifman +7 more
TL;DR: The generated dataset of PPIs—the largest collection of unbiased Coxiella host-pathogen interactions to date—represents a rich source of information with respect to secreted pathogen effector proteins and their interactions with human host proteins.
Journal ArticleDOI
Free fatty acid receptor 4 agonists induce lysophosphatidic acid receptor 1 ( LPA 1 ) desensitization independent of LPA 1 internalization and heterodimerization
Aldo Meizoso-Huesca,Socrates Villegas-Comonfort,M. Teresa Romero-Ávila,J. Adolfo García-Sáinz +3 more
TL;DR: Data indicate that FFA4 activation induces LPA1 desensitization in an internalization‐independent process and that complex cellular processes participate in the crosstalk of these receptors.
References
More filters
Journal ArticleDOI
Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages.
Maximiliano G. Gutierrez,Sharon Master,Sudha Singh,Gregory A. Taylor,María Isabel Colombo,Vojo Deretic +5 more
TL;DR: It is demonstrated that autophagic pathways can overcome the trafficking block imposed by M. tuberculosis, which is a hormonally, developmentally, and immunologically regulated process, represents an underapp appreciated innate defense mechanism for control of intracellular pathogens.
Journal ArticleDOI
Conjugative Transfer by the Virulence System of Legionella pneumophila
TL;DR: In this paper, a large number of mutants called dot that were unable to replicate intracellularly because of an inability of the bacteria to alter the endocytic pathway of macrophages were isolated.
Journal ArticleDOI
Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited.
TL;DR: Findings suggest that M. tuberculosis retards the maturation of its phagosome along the endosomal-lysosomal pathway and resides in a compartment with endosome, as opposed to lysosomal, characteristics; and the intraphagosomal pathway, i.e., the pathway followed by several intracellular parasites that inhibit phagosomes-lysOSome fusion, is heterogeneous.
Journal ArticleDOI
Phagosome maturation: aging gracefully.
TL;DR: The determinants and consequences of the fusion and fission reactions that underlie phagosomal maturation are the topic of this review.
Journal ArticleDOI
A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes.
TL;DR: It is shown that L. pneumophilaproduce a protein called RalF that functions as an exchange factor for the ADP ribosylation factor (ARF) family of guanosine triphosphatases (GTPases) and is a substrate of the Dot/Icm secretion apparatus.
Related Papers (5)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Complete genome sequence of the Q-fever pathogen Coxiella burnetii
Rekha Seshadri,Ian T. Paulsen,Ian T. Paulsen,Jonathan A. Eisen,Jonathan A. Eisen,Timothy D. Read,Karen E. Nelson,William C. Nelson,Naomi L. Ward,Naomi L. Ward,Hervé Tettelin,Tanja M. Davidsen,Maureen J. Beanan,Robert T. DeBoy,Sean C. Daugherty,Lauren M. Brinkac,Ramana Madupu,Robert J. Dodson,Hoda Khouri,K. Lee,Heather A. Carty,David J. Scanlan,Robert A. Heinzen,Herbert A. Thompson,James E. Samuel,Claire M. Fraser,Claire M. Fraser,John F. Heidelberg,John F. Heidelberg +28 more