Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii.
Daniel E. Voth,Robert A. Heinzen +1 more
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TLDR
Current understanding of the cellular events that occur during parasitism of host cells by Coxiella, including deployment of a type IV secretion system to deliver effector proteins to the host cytosol is summarized.Abstract:
Summary
Most intracellular parasites employ sophisticated mechanisms to direct biogenesis of a vacuolar replicative niche that circumvents default maturation through the endolysosomal cascade. However, this is not the case of the Q fever bacterium, Coxiella burnetii. This hardy, obligate intracellular pathogen has evolved to not only survive, but to thrive, in the harshest of intracellular compartments: the phagolysosome. Following internalization, the nascent Coxiella phagosome ultimately develops into a large and spacious parasitophorous vacuole (PV) that acquires lysosomal characteristics such as acidic pH, acid hydrolases and cationic peptides, defences designed to rid the host of intruders. However, transit of Coxiella to this environment is initially stalled, a process that is apparently modulated by interactions with the autophagic pathway. Coxiella actively participates in biogenesis of its PV by synthesizing proteins that mediate phagosome stalling, autophagic interactions, and development and maintenance of the mature vacuole. Among the potential mechanisms mediating these processes is deployment of a type IV secretion system to deliver effector proteins to the host cytosol. Here we summarize our current understanding of the cellular events that occur during parasitism of host cells by Coxiella.read more
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R-hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis-A new entry to the carrier protein family Lipocalines.
Fumihiro Motojima,Atsushi Izumi,Aem Nuylert,Zhenyu Zhai,Mohammad Dadashipour,Sayaka Shichida,Takuya Yamaguchi,Shogo Nakano,Yasuhisa Asano +8 more
TL;DR: The crystal structure and reaction mechanism of natural ChuaHNL in ligand‐free form and its complexes with acetate, cyanide ion, and inhibitors (thiocyanate or iodoacetate) at 1.6, 1.5, 2.55, and 1.55 Å resolutions are elucidated.
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Shrimp tropomyosin retains antibody reactivity after exposure to acidic condition.
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The transcriptional regulator RbcR controls ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes in the cyanobacterium Synechocystis sp. PCC 6803.
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A Unique Group I Intron in Coxiella burnetii Is a Natural Splice Mutant
TL;DR: Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3'-terminal adenine in place of a conserved guanine that has a decreased self-splicing rate in vitro.
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