Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii.
Daniel E. Voth,Robert A. Heinzen +1 more
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TLDR
Current understanding of the cellular events that occur during parasitism of host cells by Coxiella, including deployment of a type IV secretion system to deliver effector proteins to the host cytosol is summarized.Abstract:
Summary
Most intracellular parasites employ sophisticated mechanisms to direct biogenesis of a vacuolar replicative niche that circumvents default maturation through the endolysosomal cascade. However, this is not the case of the Q fever bacterium, Coxiella burnetii. This hardy, obligate intracellular pathogen has evolved to not only survive, but to thrive, in the harshest of intracellular compartments: the phagolysosome. Following internalization, the nascent Coxiella phagosome ultimately develops into a large and spacious parasitophorous vacuole (PV) that acquires lysosomal characteristics such as acidic pH, acid hydrolases and cationic peptides, defences designed to rid the host of intruders. However, transit of Coxiella to this environment is initially stalled, a process that is apparently modulated by interactions with the autophagic pathway. Coxiella actively participates in biogenesis of its PV by synthesizing proteins that mediate phagosome stalling, autophagic interactions, and development and maintenance of the mature vacuole. Among the potential mechanisms mediating these processes is deployment of a type IV secretion system to deliver effector proteins to the host cytosol. Here we summarize our current understanding of the cellular events that occur during parasitism of host cells by Coxiella.read more
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Specialization of endoplasmic reticulum chaperones for the folding and function of myelin glycoproteins P0 and PMP22
TL;DR: It is concluded that calnexin and ERp57, but not calreticulin, play an important role in the biology of peripheral myelin proteins PMP22 and P0, and, consequently, these chaperones may contribute to the pathogenesis of peripheral neuropathies and the diversity of these neurological disorders.
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Granulomatous hepatitis caused by Q fever: a differential diagnosis of fever of unknown origin.
Nancy E. Aguilar-Olivos,María del Carmen Manzano-Robleda,Ylse Gutiérrez-Grobe,Fredy Chablé-Montero,Jorge Albores-Saavedra,Eric López-Méndez +5 more
TL;DR: A 35-year-old man with FUO caused by Q fever and granulomatous hepatitis is described, with the diagnosis of acute infection by Coxiella burnetii.
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Coxiella effector protein CvpF subverts RAB26-dependent autophagy to promote vacuole biogenesis and virulence.
Fernande Ayenoue Siadous,Franck Cantet,Erin J. van Schaik,Melanie Burette,Julie Allombert,Anissa Lakhani,Boris Bonaventure,Caroline Goujon,James E. Samuel,Matteo Bonazzi,Eric Martinez +10 more
TL;DR: It is demonstrated that CBU0626 is a novel member of the Coxiella vacuolar protein (Cvp) family of effector proteins, which is translocated by the Dot/Icm secretion system and localizes to vesicles with autolysosomal features as well as Coxieella-containing vacuoles (CCVs).
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Tyrosine phosphorylation signaling regulates Ca 2+ entry by affecting intracellular pH during human sperm capacitation
Nicolás Gastón Brukman,Sol Yanel Nuñez,Lis C. Puga Molina,Mariano G. Buffone,Alberto Darszon,Patricia S. Cuasnicú,Vanina Gabriela Da Ros +6 more
TL;DR: Observations support pTyr as an intracellular sensor for Ca 2+ entry in human sperm through regulation of cytoplasmic pH.
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Molecular cloning and partial characterization of a low-density lipoprotein receptor-related protein 13 (Lrp13) involved in vitellogenin uptake in the cutthroat trout (Oncorhynchus clarki).
Yuji Mushirobira,Hiroko Mizuta,Wenshu Luo,Takashi Todo,Akihiko Hara,Benjamin J. Reading,Craig V. Sullivan,Naoshi Hiramatsu +7 more
TL;DR: Findings suggest that, in addition to lr8/LR8, l rp13/Lrp13 acts as a VtgA receptor in trout.
References
More filters
Journal ArticleDOI
Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages.
Maximiliano G. Gutierrez,Sharon Master,Sudha Singh,Gregory A. Taylor,María Isabel Colombo,Vojo Deretic +5 more
TL;DR: It is demonstrated that autophagic pathways can overcome the trafficking block imposed by M. tuberculosis, which is a hormonally, developmentally, and immunologically regulated process, represents an underapp appreciated innate defense mechanism for control of intracellular pathogens.
Journal ArticleDOI
Conjugative Transfer by the Virulence System of Legionella pneumophila
TL;DR: In this paper, a large number of mutants called dot that were unable to replicate intracellularly because of an inability of the bacteria to alter the endocytic pathway of macrophages were isolated.
Journal ArticleDOI
Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited.
TL;DR: Findings suggest that M. tuberculosis retards the maturation of its phagosome along the endosomal-lysosomal pathway and resides in a compartment with endosome, as opposed to lysosomal, characteristics; and the intraphagosomal pathway, i.e., the pathway followed by several intracellular parasites that inhibit phagosomes-lysOSome fusion, is heterogeneous.
Journal ArticleDOI
Phagosome maturation: aging gracefully.
TL;DR: The determinants and consequences of the fusion and fission reactions that underlie phagosomal maturation are the topic of this review.
Journal ArticleDOI
A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes.
TL;DR: It is shown that L. pneumophilaproduce a protein called RalF that functions as an exchange factor for the ADP ribosylation factor (ARF) family of guanosine triphosphatases (GTPases) and is a substrate of the Dot/Icm secretion apparatus.
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