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Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Journal ArticleDOI
Application of iPS cell technology to cancer epigenome study: uncovering the mechanism of cell status conversion for drug resistance in tumor.
TL;DR: The novel reprogramming technology established by Yamanaka and coworkers has made it possible for researchers to artificially introduce epigenetic remodeling into somatic cells and this technology might be able to be used as a tool to introduce the coordinated epigenetic reorganization.
BookDOI
Systems Biology in Animal Production and Health
TL;DR: This chapter proposes a pipeline using appropriate statistical tools to build a co-expression network from the list of genes, then to finely depict the network structure, and applies statistical notions linked with network theory on a reduced dataset of genes with eQTL that were found in the pig species to illustrate the basics of network inference and mining.
Journal ArticleDOI
Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.
TL;DR: A link between the removal of repressive histone H3K9 methylation and DNA methylation is uncovered during the reprogramming of somatic cells to pluripotency, which appears to be an important mechanism for altering cell identity.
Journal ArticleDOI
Reprogramming by lineage specifiers: blurring the lines between pluripotency and differentiation.
TL;DR: On the published theories for defining PSC identity, the implications that the different postulated models have for the reprogramming field as well as speculate on potential future directions that might be opened once a precise knowledge on the nature of PSCs is accomplished are discussed.
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Cell reprogramming: Into the groove
TL;DR: It is shown that growing cells on substrates with aligned features such as microgrooves can enhance the reprogramming of adult cells into pluripotent stem cells by chemical and genetic means.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.