Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
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Innate immune response precedes Mycobacterium leprae-induced reprogramming of adult Schwann cells.
Toshihiro Masaki,Aidan McGlinchey,Justyna Cholewa-Waclaw,Jinrong Qu,Simon R. Tomlinson,Anura Rambukkana +5 more
TL;DR: It is shown that reprogrammed cells possess the ability to attract macrophages, providing evidence for a functional role of immune gene products during reprogramming and suggesting a potential role of innate immune response and the related signaling pathways in cellular reprograming and the initiation of neuropathogenesis during ML infection.
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Collaborative rewiring of the pluripotency network by chromatin and signalling modulating pathways
Khoa A Tran,Steven A. Jackson,Zachariah P. G. Olufs,Nur Zafirah Zaidan,Ning Leng,Christina Kendziorski,Sushmita Roy,Rupa Sridharan +7 more
TL;DR: It is shown that combining ascorbic acid (AA) and 2i (MAP kinase and GSK inhibitors) increases the efficiency of reprogramming from fibroblasts and synergistically enhances conversion of partially reprogrammed intermediates to the iPSC state.
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Epigenetics changes associated to environmental triggers in autoimmunity.
TL;DR: An approach to the known environmental factors and the mechanisms that alter the epigenetic regulation in AIDs are showed, with emphasis in systemic lupus erythematosus, the prototype of systemic AID.
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Self-Organizing Global Gene Expression Regulated through Criticality: Mechanism of the Cell-Fate Change.
TL;DR: ‘Whole-genome’ regulation of gene expression through self-regulatory SOC control complements gene-by-gene fine tuning and represents a still largely unexplored non-equilibrium statistical mechanism that is responsible for the massive reprogramming of genome expression.
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Multiple Roles of MYC in Integrating Regulatory Networks of Pluripotent Stem Cells.
Luca Fagnocchi,Alessio Zippo +1 more
TL;DR: The MYC-controlled networks, which support the pluripotent state and discuss how their perturbation could affect cell identity are reviewed and a recent finding demonstrating a central role of MYC in triggering epigenetic memory in PSCs is discussed.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.