Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Cell reprogramming in a predictable manner on the superhydrophobic microwell array chip.
TL;DR: In this paper , a superhydrophobic microwell array chip (SMAR-chip) was used to perform iPSC induction on human cells, where cells undergo distinctive morphology change, switching from 2D monolayers to 3D clumps and develop into bona fide colonies in more than 90% of the microwells.
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MyoD-Induced Trans-Differentiation: A Paradigm for Dissecting the Molecular Mechanisms of Cell Commitment, Differentiation and Reprogramming
TL;DR: The discovery of the skeletal muscle-specific transcription factor MyoD represents a milestone in the field of transcriptional regulation during differentiation and cell-fate reprogramming as mentioned in this paper .
Journal ArticleDOI
The Role of SOX Transcription Factors in Ageing and Age-Related Diseases
TL;DR: In this paper , the authors focus on the roles of SOX transcription factors in stem cell exhaustion and age-related diseases, including neurodegenerative diseases, visual deterioration, chronic obstructive pulmonary disease, osteoporosis, and agerelated cancers.
Journal ArticleDOI
Neural stem cell research in Africa: current realities and future prospects
Idris A. Azeez,Ifeoluwa O. Awogbindin,Juliet N Olayinka,Royhaan Folarin,A. Adamu,Lydia D. Ior,Asmau M Shehu,Abubakar I Mukhtar,Olufunke F Ajeigbe,Aghariagbonse O Emokpae,Ifukibot Levi Usende,Bamidele R Babatunde,Y Yusha'u,Oladiran I. Olateju,Ronald Kamoga,Ayoola I O Benson,Kenneth C Oparaji,I O Owemidu,Musa Omoniye Iliyasu,Maryam I Imam,James O. Olopade +20 more
TL;DR: For instance, Neural Stem Cells (NSCs) are immature progenitor cells that are found in developing and adult brains that have the potential of dividing actively and renewing themselves, with a complex form of gene expression as mentioned in this paper .
Posted ContentDOI
Fibroblasts-derived from Pluripotent Cells Harboring a Single Allele Knockout in Two Pluripotency Genes Exhibit DNA Methylation Abnormalities and pluripotency induction Defects
Rachel Lasry,Noam Maoz,Albert W. Cheng,Nataly Yom Tov,Elisabeth Kulenkampff,Meir Azagury,Huanming Yang,Cora Ople,Styliani Markoulaki,Dina A. Faddah,Kirill Makedonski,Ofra Sabbag,Rudolf Jaenisch,Yosef Buganim +13 more
TL;DR: It is suggested that insufficient levels of key pluripotency genes leads to DNA methylation abnormalities in the derived-somatic cells later on in development and treatment with 5-azacytidine two days before transgene induction rescues the reprogramming defects.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
Journal ArticleDOI
Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Journal ArticleDOI
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.