Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Journal ArticleDOI
Novel Insights into Embryonic Stem Cell Self-Renewal Revealed Through Comparative Human and Mouse Systems Biology Networks
Karen G. Dowell,Allen K. Simons,Hao Bai,Braden Kell,Zack Z. Wang,Kyuson Yun,Matthew A. Hibbs +6 more
TL;DR: The molecular basis of human ESC self‐renewal is explored using Bayesian network machine learning to integrate cell‐type‐specific, high‐throughput data for gene function discovery and reflect the importance of key regulatory genes known to be strongly associated with self‐Renewal and pluripotency in both species.
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Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening
Shuyi Ji,Linying Zhu,Yimeng Gao,Xiaoran Zhang,Yupeng Yan,Jin Cen,Rongxia Li,Rong Zeng,Lujian Liao,Chunhui Hou,Yawei Gao,Shaorong Gao,Gang Wei,Lijian Hui,Lijian Hui +14 more
TL;DR: It is found that Baf60b, a member of the SWI/SNF chromatin remodeling complex, links chromatin opening to ATM activation by facilitating ATM recruitment to the open chromatin regions of a panel of hepatic gene loci.
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Epigenetics of cell fate reprogramming and its implications for neurological disorders modelling
Maciej Grzybek,Maciej Grzybek,Aleksandra Golonko,Marta Walczak,Pawel Lisowski,Pawel Lisowski +5 more
TL;DR: It is suggested that exploitation of the single-cell composition of the epigenome will provide important insights into heterogeneity within hiPSCs subpopulations to fast forward development of reliable hiPSC-based analytical platforms in neurological disorders modelling and before completed hiPSc technology will be implemented in clinical approaches.
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Gene expression of OCT4, SOX2, KLF4 and MYC (OSKM) induced pluripotent stem cells: identification for potential mechanisms
Yan-ning Cai,Yan-ning Cai,Xian-hua Dai,Xian-hua Dai,Qian-hua Zhang,Qian-hua Zhang,Zhi-ming Dai,Zhi-ming Dai +7 more
TL;DR: In this study, screened DEGs including ISG15, IRF7 and CCL5 participated in OSKM-induced pluripotency might attenuate immune response post-transduction through RLR and TLR signaling pathways.
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Reprogramming cancer cells: a novel approach for cancer therapy or a tool for disease-modeling?
TL;DR: In this review, the current knowledge on the epigenetic mechanisms involved during cancer development and cellular reprogramming will be presented, and different reports and key factors about pluripotency-based reprograming of cancer cells will be reviewed in detail.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.