Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Transcriptional Addiction in Cancer
TL;DR: How transcriptional control is disrupted by genetic alterations in cancer cells, why transcriptional dependencies can develop as a consequence of dysregulated programs, and how these dependencies provide opportunities for novel therapeutic interventions in cancer are discussed.
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A decade of transcription factor-mediated reprogramming to pluripotency
TL;DR: The mechanisms underlying transcription factor-mediated reprogramming are still poorly understood; however, several mechanistic insights have recently been obtained, making it more amenable to applications in the fields of regenerative medicine, disease modelling and drug discovery.
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Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming
Abdenour Soufi,Meilin Fernandez Garcia,Artur Jaroszewicz,Nebiyu Osman,Matteo Pellegrini,Kenneth S. Zaret +5 more
TL;DR: This work compares the nucleosome and chromatin targeting activities of Oct4, Sox2, and Klf4, which together reprogram somatic cells to pluripotency and provides insight into how pioneer factors can target naive chromatin sites.
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In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming
Alejandro Ocampo,Pradeep Reddy,Paloma Martinez-Redondo,Aida Platero-Luengo,Fumiyuki Hatanaka,Tomoaki Hishida,Mo Li,David Lam,Masakazu Kurita,Masakazu Kurita,Ergin Beyret,Toshikazu Araoka,Toshikazu Araoka,Eric Vazquez-Ferrer,David Donoso,Jose Luis Roman,Jinna Xu,Concepcion Rodriguez Esteban,Gabriel Núñez,Estrella Nuñez Delicado,Josep M. Campistol,Isabel Guillen,Pedro Guillen,Juan Carlos Izpisua Belmonte +23 more
TL;DR: It is reported that partial reprogramming by short-term cyclic expression of Oct4, Sox2, Klf4, and c-Myc (OSKM) ameliorates cellular and physiological hallmarks of aging and prolongs lifespan in a mouse model of premature aging.
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Transcription factors as readers and effectors of DNA methylation
Heng Zhu,Guohua Wang,Jiang Qian +2 more
TL;DR: Evidence is emerging to suggest that transcription factors lacking a MBD can also interact with methylated DNA, and the identification of these proteins and the elucidation of their characteristics and the biological consequences are important stepping stones towards a mechanistic understanding of methylation-mediated biological processes.
References
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The roles of the reprogramming factors Oct4, Sox2 and Klf4 in resetting the somatic cell epigenome during induced pluripotent stem cell generation
Ryan Schmidt,Kathrin Plath +1 more
TL;DR: Somatic cell reprogramming to induced pluripotent stem (iPS) cells by defined factors is a form of engineered reverse development carried out in vitro.
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Vitamin C improves the quality of somatic cell reprogramming
Miguel A. Esteban,Duanqing Pei +1 more
TL;DR: It is shown that vitamin C enhances the quality of somatic cell reprogramming in mice, highlighting the possibility that further manipulation of culture conditions could improve this technology for regenerative medicine.
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Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics.
Jiekai Chen,Jing Liu,You Chen,Jiaqi Yang,Jing Chen,He Liu,Xiangjie Zhao,Kunlun Mo,Hong Song,Lin Guo,Shilong Chu,Deping Wang,Ke Ding,Duanqing Pei +13 more
TL;DR: This work developed an optimized defined medium, iCD1, which allows Oct4/Sox2/Klf4 (OSK)-mediated reprogramming to achieve ultra-high efficiency and renders both Sox2 and Klf4 dispensable, although the elimination of these two factors leads to lower efficiency and slower kinetics.
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The RNA Pol II Elongation Factor Ell3 Marks Enhancers in ES Cells and Primes Future Gene Activation
TL;DR: For example, Ell3, a member of the Ell (eleven-nineteen lysine-rich leukemia gene) family of RNA Pol II elongation factors, occupies enhancers in embryonic stem cells as mentioned in this paper.
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Homologous Recombination DNA Repair Genes Play a Critical Role in Reprogramming to a Pluripotent State
Federico Gonzalez,Daniela Georgieva,Fabio Vanoli,Zhong-Dong Shi,Matthias Stadtfeld,Thomas Ludwig,Maria Jasin,Danwei Huangfu +7 more
TL;DR: It is shown that the ectopic expression of reprogramming factors increases the level of phosphorylated histone H2AX, one of the earliest cellular responses to DNA double-strand breaks (DSBs), which reveals a direct role of the homologous recombination (HR) pathway, a pathway essential for error-free repair of DNA DSBs, in reprograming.