Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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mTFkb: a knowledgebase for fundamental annotation of mouse transcription factors.
Kun Sun,Huating Wang,Hao Sun +2 more
TL;DR: The expression patterns of the TFs are highly correlated with the histology of the tissue types thus can be used to infer the potential functions of theTFs and a web-based database named mTFkb is built that could serve as a useful and valuable resource for TF studies in mouse.
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Large-scale mapping of mammalian transcriptomes identifies conserved genes associated with different cell states.
TL;DR: This study demonstrates that combining statistical modeling with public RNA-seq data can be powerful for improving the understanding of cell identity control.
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Bladder regeneration through stem cell therapy.
TL;DR: While autologous stem cells may provide a viable source of tissue for bladder reconstruction, their in situ activation combined with repair of the diseased microenvironment may offer better, more lasting solutions for functional bladder regeneration.
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EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.
Xianping Shi,Xianping Shi,Yueyuan Zheng,Liling Jiang,Bo Zhou,Wei Yang,Liyan Li,Ling-Wen Ding,Moli Huang,Sigal Gery,De-Chen Lin,H. Phillip Koeffler,H. Phillip Koeffler +12 more
TL;DR: Molecular characterization of the oncogenic CRC model advances the understanding of the biology of Ewing sarcoma, and indicates that CRC-downstream genes and signaling pathways may contain potential therapeutic targets for this malignancy.
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Generation of porcine induced-pluripotent stem cells from Sertoli cells.
Piyathip Setthawong,Praopilas Phakdeedindan,Narong Tiptanavattana,Sasitorn Rungarunlert,Mongkol Techakumphu,Theerawat Tharasanit +5 more
TL;DR: Sertoli cells can efficiently serve as a novel cell source for iPSC reprogramming and two cell lines used for in vivo differentiation produced high-efficiency teratoma.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Journal ArticleDOI
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.