Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Journal ArticleDOI
Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Hana Benchetrit,Mohammad Jaber,Valery Zayat,Shulamit Sebban,Avital Pushett,Kirill Makedonski,Zvi Zakheim,Ahmed Radwan,Noam Maoz,Rachel Lasry,Noa Renous,Michal Inbar,Oren Ram,Tommy Kaplan,Yosef Buganim +14 more
TL;DR: Mechanistically, it is shown that the interplay between Esrrb and Eomes during the reprogramming process determines cell fate, where high levels of EsRRb induce a XEN-like state that drives pluripotency and high Levels of Eomes drive trophectodermal fate.
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Cell engineering: Biophysical regulation of the nucleus.
TL;DR: This review will highlight the recent progress in nuclear biomechanics and mechanobiology in the context of cell engineering, tissue remodeling and disease development.
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Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.
Khoa A Tran,Stefan J. Pietrzak,Nur Zafirah Zaidan,Alireza Fotuhi Siahpirani,Sunnie Grace McCalla,Amber S. Zhou,Gopal Iyer,Sushmita Roy,Rupa Sridharan +8 more
TL;DR: A critical role for signaling inhibition by 2i in repressing somatic expression is identified and synergy between the epigenomic modifiers ascorbic acid and a Dot1L inhibitor for pluripotency gene activation is identified.
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Transcription factor-mediated reprogramming toward hematopoietic stem cells
TL;DR: This review will integrate the recently reported strategies to directly convert a variety of starting cell types toward HSCs in the context of hematopoietic transcriptional regulation and discuss how these findings could be further developed toward the ultimate generation of therapeutic human H SCs.
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Methylated cis-regulatory elements mediate KLF4-dependent gene transactivation and cell migration.
Jun Wan,Yijing Su,Qifeng Song,Brian Tung,Brian Tung,Olutobi Oyinlade,Sheng Liu,Mingyao Ying,Mingyao Ying,Guo Li Ming,Hongjun Song,Jiang Qian,Heng Zhu,Shuli Xia +13 more
TL;DR: The biological function of mCpG-dependent gene regulation by KLF4 in glioblastoma cells is reported, demonstrating a new paradigm of DNA methylation-mediated gene activation and chromatin remodeling, and providing a general framework to dissect the biological functions ofDNA methylation readers and effectors.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.