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Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Antiviral Responses in Mouse Embryonic Stem Cells: Differential Development of Cellular Mechanisms in Type I Interferon Production and Response
TL;DR: It is reported that mESCs are able to respond to type I IFNs, express IFN-stimulated genes, and mediate the antiviral effect of type IIFNs against La Crosse virus and chikungunya virus.
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Reprogramming away from the exhausted T cell state.
TL;DR: Cell reprogramming using iPSC technologies aims to overcome the problem by returning TILs to the pluripotent state from which they can be differentiated into a heterogeneous population of T cells that are best suited to combat the tumor.
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Blockade of senescence‐associated microRNA‐195 in aged skeletal muscle cells facilitates reprogramming to produce induced pluripotent stem cells.
TL;DR: Novel evidence is provided that the blockade of age‐induced miR‐195 is a promising approach for efficient iPSC generation from aging donor subjects, which has the potential for autologous transplantation of iPSCs in elderly patients.
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Intrinsic and extrinsic molecular determinants or modulators for epigenetic remodeling and reprogramming of somatic cell-derived genome in mammalian nuclear-transferred oocytes and resultant embryos.
Marcin Samiec,Maria Skrzyszowska +1 more
TL;DR: Epigenomic modulation of nuclear donor cells, nuclear recipient cells and/or cloned embryos may facilitate and accelerate the reprogrammability for gene expression of donor cell nuclei that have been transplanted into a host ooplasm and subsequently underwent dedifferentiating and re-establishing the epigenetically dependent status of their transcriptional activity during pre- and postimplantation development of NT embryos.
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Engineering Biomaterials with Micro/Nanotechnologies for Cell Reprogramming
Jun Fang,Yuan Yu Hsueh,Yuan Yu Hsueh,Jennifer Soto,Wujin Sun,Jinqiang Wang,Zhen Gu,Ali Khademhosseini,Song Li +8 more
TL;DR: An overview of the state-of-the-art technologies for cell reprograming is presented and the recent breakthroughs in engineering biomaterials with micro/nanotechnologies to improve reprogramming efficiency and quality are highlighted.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.