Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Transcriptional Addiction in Cancer
TL;DR: How transcriptional control is disrupted by genetic alterations in cancer cells, why transcriptional dependencies can develop as a consequence of dysregulated programs, and how these dependencies provide opportunities for novel therapeutic interventions in cancer are discussed.
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A decade of transcription factor-mediated reprogramming to pluripotency
TL;DR: The mechanisms underlying transcription factor-mediated reprogramming are still poorly understood; however, several mechanistic insights have recently been obtained, making it more amenable to applications in the fields of regenerative medicine, disease modelling and drug discovery.
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Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming
Abdenour Soufi,Meilin Fernandez Garcia,Artur Jaroszewicz,Nebiyu Osman,Matteo Pellegrini,Kenneth S. Zaret +5 more
TL;DR: This work compares the nucleosome and chromatin targeting activities of Oct4, Sox2, and Klf4, which together reprogram somatic cells to pluripotency and provides insight into how pioneer factors can target naive chromatin sites.
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In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming
Alejandro Ocampo,Pradeep Reddy,Paloma Martinez-Redondo,Aida Platero-Luengo,Fumiyuki Hatanaka,Tomoaki Hishida,Mo Li,David Lam,Masakazu Kurita,Masakazu Kurita,Ergin Beyret,Toshikazu Araoka,Toshikazu Araoka,Eric Vazquez-Ferrer,David Donoso,Jose Luis Roman,Jinna Xu,Concepcion Rodriguez Esteban,Gabriel Núñez,Estrella Nuñez Delicado,Josep M. Campistol,Isabel Guillen,Pedro Guillen,Juan Carlos Izpisua Belmonte +23 more
TL;DR: It is reported that partial reprogramming by short-term cyclic expression of Oct4, Sox2, Klf4, and c-Myc (OSKM) ameliorates cellular and physiological hallmarks of aging and prolongs lifespan in a mouse model of premature aging.
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Transcription factors as readers and effectors of DNA methylation
Heng Zhu,Guohua Wang,Jiang Qian +2 more
TL;DR: Evidence is emerging to suggest that transcription factors lacking a MBD can also interact with methylated DNA, and the identification of these proteins and the elucidation of their characteristics and the biological consequences are important stepping stones towards a mechanistic understanding of methylation-mediated biological processes.
References
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Reprogramming Factor Stoichiometry Influences the Epigenetic State and Biological Properties of Induced Pluripotent Stem Cells
Bryce W. Carey,Styliani Markoulaki,Jacob H. Hanna,Dina A. Faddah,Yosef Buganim,Jongpil Kim,Kibibi Ganz,Eveline J. Steine,John P. Cassady,Menno P. Creyghton,G. Grant Welstead,Qing Gao,Rudolf Jaenisch +12 more
TL;DR: The data indicate that stoichiometry of reprogramming factors can influence epigenetic and biological properties of iPS cells and should be considered when comparing different iPS and ES cell lines.
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Tbx3 improves the germ-line competency of induced pluripotent stem cells
Jianyong Han,Ping Yuan,Henry Yang,Jinqiu Zhang,Boon Seng Soh,Pin Li,Siew Lan Lim,Suying Cao,Junliang Tay,Yuriy L. Orlov,Thomas Lufkin,Huck-Hui Ng,Wai Leong Tam,Bing Lim +13 more
TL;DR: iPS cells generated with OSK and Tbx3 (OSKT) are superior in both germ-cell contribution to the gonads and germ-line transmission frequency, and the need to rigorously characterize iPS cells beyond in vitro studies is highlighted.
Journal ArticleDOI
Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells.
Kazim H. Narsinh,Ning Sun,Veronica Sanchez-Freire,Andrew S. Lee,Patrícia de Almeida,Shijun Hu,Taha A. Jan,Kitchener D. Wilson,Denise Leong,Jarrett Rosenberg,Mylene Yao,Robert C. Robbins,Joseph C. Wu +12 more
TL;DR: It is suggested that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims, because of single cell heterogeneity amongst stem cell populations.
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Proteomic and phosphoproteomic comparison of human ES and iPS cells
Douglas H. Phanstiel,Justin Brumbaugh,Justin Brumbaugh,Craig D. Wenger,Shulan Tian,Mitchell D. Probasco,Derek J. Bailey,Danielle L. Swaney,Mark A. Tervo,Jennifer M. Bolin,Victor Ruotti,Ron Stewart,James A. Thomson,James A. Thomson,James A. Thomson,Joshua J. Coon +15 more
TL;DR: Deep proteomic coverage of four human embryonic stem cell and four induced pluripotent stem cell lines in biological triplicate is reported and the Stem Cell–Omics Repository is introduced, a resource to collate and display quantitative information across multiple planes of measurement, including mRNA, protein and post-translational modifications.
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MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition.
Baojian Liao,Xichen Bao,Xichen Bao,Longqi Liu,Shipeng Feng,Athanasios Zovoilis,Wenbo Liu,Yanting Xue,Jie Cai,Xiangpeng Guo,Baoming Qin,Ruosi Zhang,Jiayan Wu,Liangxue Lai,Maikun Teng,Liwen Niu,Biliang Zhang,Miguel A. Esteban,Duanqing Pei +18 more
TL;DR: This work has successfully delivered entire miRNA clusters into reprogramming fibroblasts using retroviral vectors and adds new evidence for the emerging relationship between pluripotency and the epithelial phenotype.