Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
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NF-κB activation impairs somatic cell reprogramming in ageing
Clara Soria-Valles,Fernando G. Osorio,Ana Gutiérrez-Fernández,Alejandro De Los Angeles,Clara Bueno,Pablo Menendez,José I. Martín-Subero,George Q. Daley,José M.P. Freije,Carlos López-Otín +9 more
TL;DR: It is demonstrated that DOT1L inhibition in vivo extends lifespan and ameliorates the accelerated ageing phenotype of progeroid mice, supporting the interest of studying age-associated molecular impairments to identify targets of rejuvenation strategies.
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p53: The barrier to cancer stem cell formation
TL;DR: The role of p53 in genomically stable embryonicstem cells, a unique predisposed cancer stem cell model and adult stem cells, its role in the generation of induced pluripotent stem cells is addressed, as well as its role as the barrier to cancer stem cells formation is addressed.
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Quantification of Retinogenesis in 3D Cultures Reveals Epigenetic Memory and Higher Efficiency in iPSCs Derived from Rod Photoreceptors.
Daniel J. Hiler,Xiang Chen,Jennifer L. Hazen,Sergey Kupriyanov,Patrick A. Carroll,Chunxu Qu,Beisi Xu,Dianna A. Johnson,Lyra Griffiths,Sharon Frase,Alberto R. Rodriguez,Greg S. Martin,Jiakun Zhang,Jongrye Jeon,Yiping Fan,David Finkelstein,Robert N. Eisenman,Kristin K. Baldwin,Michael A. Dyer,Michael A. Dyer,Michael A. Dyer +20 more
TL;DR: It is suggested that the source of stem cells is important for producing retinal neurons in three-dimensional (3D) organ cultures.
Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution
TL;DR: In this paper, the authors established a reporter of genomic methylation (RGM) that relies on a minimal imprinted gene promoter driving a fluorescent protein, and showed that insertion of RGM proximal to promoter-associated CpG islands reports the gain or loss of DNA methylation.
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Proteins that bind regulatory regions identified by histone modification chromatin immunoprecipitations and mass spectrometry
Erik Engelen,Johannes H. Brandsma,Maaike J. Moen,Luca Signorile,Dick H. W. Dekkers,Jeroen Demmers,Christel E M Kockx,Zehila Ozgür,Wilfred F. J. van IJcken,Debbie L. C. van den Berg,Raymond A. Poot +10 more
TL;DR: The ChIP-MS method provides a detailed read-out of the transcriptional landscape representative of the investigated cell type and determines the genome-wide binding sites of Dppa2, which is found to operate outside the classical pluripotency network.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.