Open Access
Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
Citations
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Journal ArticleDOI
Chromatin Accessibility Dynamics during iPSC Reprogramming
Dongwei Li,Jing Liu,Xuejie Yang,Chunhua Zhou,Jing Guo,Chuman Wu,Yue Qin,Lin Guo,Jiangping He,Shenyong Yu,He Liu,Xiaoshan Wang,Fang Wu,Junqi Kuang,Andrew P. Hutchins,Jiekai Chen,Duanqing Pei +16 more
TL;DR: In this article, ATAC-seq profiling of MEFs expressing Oct4-Sox2-Klf4 (OSK) reveals dynamic changes in chromatin states shifting from open to closed (OC) and closed to open (CO), with an initial burst of OC and an ending surge of CO.
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Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming
Peter J. Quesenberry,Jason M. Aliotta,Maria Chiara Deregibus,Maria Chiara Deregibus,Giovanni Camussi +4 more
TL;DR: This review focuses on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications and the relevance of these phenomena in stem cell biology and tissue repair.
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A Systematic Approach to Identify Candidate Transcription Factors that Control Cell Identity.
Ana C. D’Alessio,Zi Peng Fan,Katherine J. Wert,Petr Baranov,Malkiel A. Cohen,Janmeet S. Saini,Janmeet S. Saini,Evan Cohick,Carol Charniga,Daniel B. Dadon,Nancy M. Hannett,Michael J. Young,Sally Temple,Rudolf Jaenisch,Tong Ihn Lee,Richard A. Young +15 more
TL;DR: It is suggested that candidate core TFs from the atlas will prove a useful starting point for studying transcriptional control of cell identity and reprogramming in many human cell types.
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Ten years of progress and promise of induced pluripotent stem cells: historical origins, characteristics, mechanisms, limitations, and potential applications
TL;DR: The progress and the recent advances that have been made over the last 10 years in the iPSC field are summarized, with emphasis on the molecular mechanism of reprogramming, strategies to improve the efficiency of repprogramming, characteristics and limitations of iPSCs, and the progress made in the applications ofiPSCs in the field of disease modelling, drug discovery and regenerative medicine.
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OCT4: dynamic DNA binding pioneers stem cell pluripotency.
TL;DR: An integrated view of OCT4 research conducted to date is provided by reviewing the different functional roles for OCT4 and discussing the current progress in understanding their underlying molecular mechanisms.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.