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Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
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Transcriptional Addiction in Cancer
TL;DR: How transcriptional control is disrupted by genetic alterations in cancer cells, why transcriptional dependencies can develop as a consequence of dysregulated programs, and how these dependencies provide opportunities for novel therapeutic interventions in cancer are discussed.
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A decade of transcription factor-mediated reprogramming to pluripotency
TL;DR: The mechanisms underlying transcription factor-mediated reprogramming are still poorly understood; however, several mechanistic insights have recently been obtained, making it more amenable to applications in the fields of regenerative medicine, disease modelling and drug discovery.
Journal ArticleDOI
Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming
Abdenour Soufi,Meilin Fernandez Garcia,Artur Jaroszewicz,Nebiyu Osman,Matteo Pellegrini,Kenneth S. Zaret +5 more
TL;DR: This work compares the nucleosome and chromatin targeting activities of Oct4, Sox2, and Klf4, which together reprogram somatic cells to pluripotency and provides insight into how pioneer factors can target naive chromatin sites.
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In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming
Alejandro Ocampo,Pradeep Reddy,Paloma Martinez-Redondo,Aida Platero-Luengo,Fumiyuki Hatanaka,Tomoaki Hishida,Mo Li,David Lam,Masakazu Kurita,Masakazu Kurita,Ergin Beyret,Toshikazu Araoka,Toshikazu Araoka,Eric Vazquez-Ferrer,David Donoso,Jose Luis Roman,Jinna Xu,Concepcion Rodriguez Esteban,Gabriel Núñez,Estrella Nuñez Delicado,Josep M. Campistol,Isabel Guillen,Pedro Guillen,Juan Carlos Izpisua Belmonte +23 more
TL;DR: It is reported that partial reprogramming by short-term cyclic expression of Oct4, Sox2, Klf4, and c-Myc (OSKM) ameliorates cellular and physiological hallmarks of aging and prolongs lifespan in a mouse model of premature aging.
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Transcription factors as readers and effectors of DNA methylation
Heng Zhu,Guohua Wang,Jiang Qian +2 more
TL;DR: Evidence is emerging to suggest that transcription factors lacking a MBD can also interact with methylated DNA, and the identification of these proteins and the elucidation of their characteristics and the biological consequences are important stepping stones towards a mechanistic understanding of methylation-mediated biological processes.
References
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Journal ArticleDOI
Direct cell reprogramming is a stochastic process amenable to acceleration
Jacob H. Hanna,Krishanu Saha,Bernardo F. Pando,Jeroen S. van Zon,Christopher J. Lengner,Menno P. Creyghton,Alexander van Oudenaarden,Rudolf Jaenisch +7 more
TL;DR: Quantitative analyses define distinct cell-division-rate-dependent and -independent modes for accelerating the stochastic course of reprogramming, and suggest that the number of cell divisions is a key parameter driving epigenetic reprograming to pluripotency.
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Hyperdynamic Plasticity of Chromatin Proteins in Pluripotent Embryonic Stem Cells
Eran Meshorer,Dhananjay Yellajoshula,Eric M. George,Peter J. Scambler,David T. Brown,Tom Misteli +5 more
TL;DR: It is suggested that hyperdynamic binding of structural chromatin proteins is a functionally important hallmark of pluripotent ES cells that contributes to the maintenance of plasticity in undifferentiated ES cells and to establishing higher-order chromatin structure.
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c-Myc Is a Universal Amplifier of Expressed Genes in Lymphocytes and Embryonic Stem Cells
Zuqin Nie,Gangqing Hu,Gang Wei,Kairong Cui,Arito Yamane,Wolfgang Resch,Ruoning Wang,Douglas R. Green,Lino Tessarollo,Rafael Casellas,Keji Zhao,David Levens +11 more
TL;DR: To observe Myc target expression and function in a system where Myc is temporally and physiologically regulated, the transcriptomes and the genome-wide distributions of Myc, RNA polymerase II, and chromatin modifications were compared during lymphocyte activation and in ES cells as well.
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Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines
Christoph Bock,Evangelos Kiskinis,Evangelos Kiskinis,Griet Verstappen,Griet Verstappen,Hongcang Gu,Gabriella L. Boulting,Zachary D. Smith,Zachary D. Smith,Michael J. Ziller,Michael J. Ziller,Gist F. Croft,Mackenzie W. Amoroso,Derek H. Oakley,Andreas Gnirke,Kevin Eggan,Kevin Eggan,Alexander Meissner,Alexander Meissner +18 more
TL;DR: This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines, and yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
Journal ArticleDOI
Copy number variation and selection during reprogramming to pluripotency
Samer M.I. Hussein,Nizar N. Batada,Sanna Vuoristo,Reagan W. Ching,Reija Autio,Reija Autio,Elisa Närvä,Siemon Ng,Michel Sourour,Riikka H. Hämäläinen,Cia Olsson,Karolina Lundin,Milla Mikkola,Ras Trokovic,Michael Peitz,Oliver Brüstle,David P. Bazett-Jones,Kari Alitalo,Riitta Lahesmaa,Andras Nagy,Timo Otonkoski +20 more
TL;DR: It is shown that significantly more CNVs are present in early-passage human iPS cells than intermediate passage human i PS cells, fibroblast cell origins and with human embryonic stem (ES) cells.