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Mechanisms and models of somatic cell reprogramming
TLDR
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) as discussed by the authors ) was the first recipient of the WBIR grant. But this work was performed in a supervised setting.Abstract:
Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship)read more
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Tet3-Mediated DNA Demethylation Contributes to the Direct Conversion of Fibroblast to Functional Neuron.
Juan Zhang,Shuangquan Chen,Dongming Zhang,Zixiao Shi,Hong Li,Tongbiao Zhao,Baoyang Hu,Qi Zhou,Jianwei Jiao +8 more
TL;DR: It is shown that Tet3, a DNA dioxygenase, can rapidly and efficiently convert fibroblasts directly into functional neurons, suggesting that DNA demethylation may regulate direct lineage commitment, representing an avenue for investigating the process of transdifferentiation.
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Micro-management of pluripotent stem cells.
TL;DR: Systematic screening for novel miRNAs that regulate the establishment and maintenance of pluripotent stem cells and further mechanistic investigations will not only shed new light on the biology of ESCs and iPSCs, but also help develop safe and efficient technologies to manipulate cell fate for regenerative medicine.
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The Chromatin Signature of Pluripotency: Establishment and Maintenance.
TL;DR: This review focuses on the chromatin and epigenetic characteristics that distinguish stem cells from somatic cells and their dynamic changes during differentiation and reprogramming.
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The fate of cell reprogramming
TL;DR: The ability to convert somatic cells to induced pluripotent stem cells has immense potential to further the understanding of development and disease mechanisms, and for cellular therapy.
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MYC Releases Early Reprogrammed Human Cells from Proliferation Pause via Retinoblastoma Protein Inhibition.
Tim A. Rand,Kenta Sutou,Koji Tanabe,Daeun Jeong,Masaki Nomura,Fumiyo Kitaoka,Emi Tomoda,Megumi Narita,Michiko Nakamura,Masahiro Nakamura,Akira Watanabe,Eric Rulifson,Shinya Yamanaka,Shinya Yamanaka,Kazutoshi Takahashi +14 more
TL;DR: It is reported that MYC rescues early human cells undergoing reprogramming from a proliferation pause induced by OCT3/4, SOX2, and KLF4 (OSK), and identified ESRG as a marker of early reprograming cells that is expressed as early as day 3 after OSK induction.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang,Tao Liu,Clifford A. Meyer,Jérôme Eeckhoute,David S. Johnson,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,Richard M. Myers,Myles Brown,Wei Li,X. Shirley Liu +11 more
TL;DR: This work presents Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's Genome Analyzer, and uses a dynamic Poisson distribution to effectively capture local biases in the genome, allowing for more robust predictions.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.