MicroRNAs in plasma of pancreatic ductal adenocarcinoma patients as novel blood-based biomarkers of disease
Jin Wang,Jinyun Chen,Ping Chang,Aimee Leblanc,Donghui Li,James L. Abbruzzesse,Marsha L. Frazier,Ann M. Killary,Subrata Sen +8 more
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TLDR
Observations show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the future.Abstract:
Development of minimally invasive biomarker assays for early detection and effective clinical management of pancreatic cancer is urgently needed to reduce high morbidity and mortality associated with this malignancy We hypothesized that if aberrantly expressing microRNAs (miRNA) in pancreatic adenocarcinoma tissues are detected in blood plasma, then plasma profiling of these miRNAs might serve as a minimally invasive early detection biomarker assay for this malignancy By using a modified protocol to isolate and quantify plasma miRNAs from heparin-treated blood, we show that miRNA profiling in plasma can differentiate pancreatic adenocarcinoma patients from healthy controls We have profiled four miRNAs, miR-21, miR-210, miR-155, and miR-196a, all implicated in the development of pancreatic cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways Of these, miR-155 has recently been identified as a candidate biomarker of early pancreatic neoplasia, whereas elevated expression of miR196a has been shown to parallel progression of disease The results revealed a sensitivity of 64% and a specificity of 89% with the analyses of plasma levels for this panel of four miRNAs The area under the receiver operating characteristic curve were estimated at 082 and 078 without and with leave-one-out cross-validation scheme, respectively These observations, although a "proof of principle" finding at this time, show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the futureread more
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Prognostic MicroRNAs in Cancer Tissue from Patients Operated for Pancreatic Cancer—Five MicroRNAs in a Prognostic Index
Nicolai Aagaard Schultz,Klaus Kaae Andersen,Anne Roslind,Hanni Willenbrock,Morten Wøjdemann,Julia S. Johansen +5 more
TL;DR: The combination of five miRNAs expression in non micro-dissected FFPE PC tissue can identify patients with short OS after radical surgery, independent of chemotherapy treatment.
Journal ArticleDOI
MicroRNA functional network in pancreatic cancer: From biology to biomarkers of disease
Jin Wang,Subrata Sen +1 more
TL;DR: How the oncogenic miRs may be involved in the genetic networks regulating functional pathways deregulated in this malignancy is discussed.
Journal ArticleDOI
Diagnostic and biological significance of microRNA-192 in pancreatic ductal adenocarcinoma
Chen-Yan Zhao,Jing Zhang,Shuhui Zhang,Danghui Yu,Ying Chen,Qing-Hua Liu,Min Shi,Can-Rong Ni,Minghua Zhu +8 more
TL;DR: Serum microRNA-192 (miR-192) may serve as a sensitive diagnostic biomarker for PDAC, which is associated with repression of SIP1 and alteration of cell cycle regulatory genes.
Journal ArticleDOI
Prognostic impact of circulating miR-21 and miR-375 in plasma of patients with esophageal squamous cell carcinoma
Shuhei Komatsu,Daisuke Ichikawa,Hiroki Takeshita,Hirotaka Konishi,Hiroaki Nagata,Shoji Hirajima,Tsutomu Kawaguchi,Tomohiro Arita,Atsushi Shiozaki,Hitoshi Fujiwara,Kazuma Okamoto,Eigo Otsuji +11 more
TL;DR: CirculatingmiR-21 and miR-375 could be reliable prognostic markers for ESCC and facilitate clinical decision-making to select prospective candidates, which need meticulous follow-up for early detection of recurrences and additional treatments such as neo-adjuvant chemotherapy and postoperative chemotherapy in ESCC.
Journal ArticleDOI
Recent progress toward the use of circulating microRNAs as clinical biomarkers.
Vikas Ghai,Kai Wang +1 more
TL;DR: The current state of miRNA biomarkers for many human diseases, including their emerging use in toxicological applications, are reviewed and the current challenges in the field are discussed, with an emphasis on technical issues that often hinder discovery-based mi RNA biomarker studies.
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A microRNA expression signature of human solid tumors defines cancer gene targets
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