MicroRNAs in plasma of pancreatic ductal adenocarcinoma patients as novel blood-based biomarkers of disease
Jin Wang,Jinyun Chen,Ping Chang,Aimee Leblanc,Donghui Li,James L. Abbruzzesse,Marsha L. Frazier,Ann M. Killary,Subrata Sen +8 more
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TLDR
Observations show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the future.Abstract:
Development of minimally invasive biomarker assays for early detection and effective clinical management of pancreatic cancer is urgently needed to reduce high morbidity and mortality associated with this malignancy We hypothesized that if aberrantly expressing microRNAs (miRNA) in pancreatic adenocarcinoma tissues are detected in blood plasma, then plasma profiling of these miRNAs might serve as a minimally invasive early detection biomarker assay for this malignancy By using a modified protocol to isolate and quantify plasma miRNAs from heparin-treated blood, we show that miRNA profiling in plasma can differentiate pancreatic adenocarcinoma patients from healthy controls We have profiled four miRNAs, miR-21, miR-210, miR-155, and miR-196a, all implicated in the development of pancreatic cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways Of these, miR-155 has recently been identified as a candidate biomarker of early pancreatic neoplasia, whereas elevated expression of miR196a has been shown to parallel progression of disease The results revealed a sensitivity of 64% and a specificity of 89% with the analyses of plasma levels for this panel of four miRNAs The area under the receiver operating characteristic curve were estimated at 082 and 078 without and with leave-one-out cross-validation scheme, respectively These observations, although a "proof of principle" finding at this time, show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the futureread more
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Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs
Chenguang Wang,Huan Zhang,Dongdong Zeng,Wenliang Sun,Honglu Zhang,Ali Aldalbahi,Yunsheng Wang,Lili San,Chunhai Fan,Xiaolei Zuo,Mi Xianqiang +10 more
TL;DR: This work demonstrated a novel strategy for the detection of multiple microRNAs (miRNAs) based on gold nanoparticles and polyadenine mediated nanoscale molecular beacon probes and achieved high selectivity for specifically distinguishing DNA targets from single-base mismatched DNA targets.
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Downregulation of miR-124 predicts poor prognosis in pancreatic ductal adenocarcinoma patients
TL;DR: Serum miR-124 levels have utility as diagnostic biomarkers in patients with PDAC, and for the first time, it is suggested that serum miR -124 levels may have prognostic impact in patientsWith PDAC.
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Systematic review/Meta-analysis Diagnostic value of microRNA for pancreatic cancer: a meta-analysis
TL;DR: The results of microRNA assays should be interpreted in parallel with clinical findings and the results of conventional tests, as they play an important role in the diagnosis of pancreatic cancer.
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Emerging roles of microRNAs in pancreatic cancer diagnosis, therapy and prognosis (Review)
Ramadevi Subramani,Laxman Gangwani,Sushmita Bose Nandy,Arunkumar Arumugam,Munmun Chattopadhyay,Rajkumar Lakshmanaswamy +5 more
TL;DR: Identification of key miRNA networks in pancreatic cancer will provide long-awaited diagnostic/therapeutic/prognostic tools for early detection, better treatment options, and extended life expectancy and quality of life in PDAC patients.
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Chemoresistance, Cancer Stem Cells, and miRNA Influences: The Case for Neuroblastoma
Alfred Buhagiar,Duncan Ayers +1 more
TL;DR: This review focuses on highlighting the recent, important implications of miRNAs within the context of neuroblastoma basic research efforts, particularly concerning miRNA influences on cancer stem cell pathology and chemoresistance pathology for this condition, together with development of translational medicine approaches for novel diagnostic tools and therapies for this Neuroblastoma.
References
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Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
TL;DR: It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.
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A microRNA expression signature of human solid tumors defines cancer gene targets
Stefano Volinia,George A. Calin,Chang Gong Liu,Stefan Ambs,Amelia Cimmino,Fabio Petrocca,Rosa Visone,Marilena V. Iorio,Claudia Roldo,Manuela Ferracin,Robyn L. Prueitt,Nozumu Yanaihara,Giovanni Lanza,Aldo Scarpa,Andrea Vecchione,Massimo Negrini,Curtis C. Harris,Carlo M. Croce +17 more
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MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells.
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