MicroRNAs in plasma of pancreatic ductal adenocarcinoma patients as novel blood-based biomarkers of disease
Jin Wang,Jinyun Chen,Ping Chang,Aimee Leblanc,Donghui Li,James L. Abbruzzesse,Marsha L. Frazier,Ann M. Killary,Subrata Sen +8 more
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TLDR
Observations show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the future.Abstract:
Development of minimally invasive biomarker assays for early detection and effective clinical management of pancreatic cancer is urgently needed to reduce high morbidity and mortality associated with this malignancy We hypothesized that if aberrantly expressing microRNAs (miRNA) in pancreatic adenocarcinoma tissues are detected in blood plasma, then plasma profiling of these miRNAs might serve as a minimally invasive early detection biomarker assay for this malignancy By using a modified protocol to isolate and quantify plasma miRNAs from heparin-treated blood, we show that miRNA profiling in plasma can differentiate pancreatic adenocarcinoma patients from healthy controls We have profiled four miRNAs, miR-21, miR-210, miR-155, and miR-196a, all implicated in the development of pancreatic cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways Of these, miR-155 has recently been identified as a candidate biomarker of early pancreatic neoplasia, whereas elevated expression of miR196a has been shown to parallel progression of disease The results revealed a sensitivity of 64% and a specificity of 89% with the analyses of plasma levels for this panel of four miRNAs The area under the receiver operating characteristic curve were estimated at 082 and 078 without and with leave-one-out cross-validation scheme, respectively These observations, although a "proof of principle" finding at this time, show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the futureread more
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Serum microRNA-195 is down-regulated in breast cancer: a potential marker for the diagnosis of breast cancer
TL;DR: Serum miR-195 is a promising tumor marker for BC diagnosis and general screening, especially for early stage BC, and has a higher sensitivity for detecting the response to neoadjuvant chemotherapy and significantly increased, more than twofold, after neoad juvant chemotherapy.
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MicroRNA in pancreatic adenocarcinoma: predictive/prognostic biomarkers or therapeutic targets?
Oronzo Brunetti,Antonio Russo,Aldo Scarpa,Daniele Santini,Michele Reni,Alessandro Bittoni,Amalia Azzariti,Giuseppe Aprile,Sabina Delcuratolo,Michele Signorile,Antonio Gnoni,Loredana Palermo,Vito Lorusso,Stefano Cascinu,Nicola Silvestris +14 more
TL;DR: It is the opinion that the preclinical evidence of the role of specific miRNAs in carcinogenesis, the possibility of using mi RNAs as diagnostic or prognostic biomarkers, and their potential therapeutic role, warrant future studies in PDAC.
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Circulating U2 Small Nuclear RNA Fragments as a Novel Diagnostic Tool for Patients with Epithelial Ovarian Cancer
Jan Dominik Kuhlmann,Alexander Baraniskin,Stephan A. Hahn,Frank Mosel,Maren Bredemeier,Pauline Wimberger,Rainer Kimmig,Sabine Kasimir-Bauer +7 more
TL;DR: This is the first report to suggest that a circulating snRNA can serve as an auxiliary diagnostic tool for monitoring tumor dynamics in ovarian cancer, and the results provide a rationale to further investigate whether this high-risk patient group may benefit from additional therapies that are directly applied after chemotherapy.
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Liquid biopsy in patients with pancreatic cancer: Circulating tumor cells and cell-free nucleic acids.
Taisuke Imamura,Shuhei Komatsu,Daisuke Ichikawa,Tsutomu Kawaguchi,Mahito Miyamae,Wataru Okajima,Takuma Ohashi,Tomohiro Arita,Hirotaka Konishi,Atsushi Shiozaki,Ryo Morimura,Hisashi Ikoma,Kazuma Okamoto,Eigo Otsuji +13 more
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