MicroRNAs in plasma of pancreatic ductal adenocarcinoma patients as novel blood-based biomarkers of disease
Jin Wang,Jinyun Chen,Ping Chang,Aimee Leblanc,Donghui Li,James L. Abbruzzesse,Marsha L. Frazier,Ann M. Killary,Subrata Sen +8 more
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TLDR
Observations show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the future.Abstract:
Development of minimally invasive biomarker assays for early detection and effective clinical management of pancreatic cancer is urgently needed to reduce high morbidity and mortality associated with this malignancy We hypothesized that if aberrantly expressing microRNAs (miRNA) in pancreatic adenocarcinoma tissues are detected in blood plasma, then plasma profiling of these miRNAs might serve as a minimally invasive early detection biomarker assay for this malignancy By using a modified protocol to isolate and quantify plasma miRNAs from heparin-treated blood, we show that miRNA profiling in plasma can differentiate pancreatic adenocarcinoma patients from healthy controls We have profiled four miRNAs, miR-21, miR-210, miR-155, and miR-196a, all implicated in the development of pancreatic cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways Of these, miR-155 has recently been identified as a candidate biomarker of early pancreatic neoplasia, whereas elevated expression of miR196a has been shown to parallel progression of disease The results revealed a sensitivity of 64% and a specificity of 89% with the analyses of plasma levels for this panel of four miRNAs The area under the receiver operating characteristic curve were estimated at 082 and 078 without and with leave-one-out cross-validation scheme, respectively These observations, although a "proof of principle" finding at this time, show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the futureread more
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miR-196a overexpression and miR-196a2 gene polymorphism are prognostic predictors of oral carcinomas
Chung Ji Liu,Meng Miao Tsai,Hsi Feng Tu,Man Tin Lui,Man Tin Lui,Hui Wen Cheng,Shu Chun Lin,Shu Chun Lin +7 more
TL;DR: Multivariate analysis showed that both high miR-196a expression and TT genotype were independent predictors for poor survival in OSCC, and the risk of mortality was greatest for patients with highMiR- 196a level and positive node status.
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Knockdown of microRNA-21 Inhibits Proliferation and Increases Cell Death by Targeting Programmed Cell Death 4 (PDCD4) in Pancreatic Ductal Adenocarcinoma
Imran Bhatti,Andrew Lee,Victoria James,Richard I. Hall,Jonathan N. Lund,Cristina Tufarelli,Dileep N. Lobo,Michael Larvin +7 more
TL;DR: Examination of expression of a panel of five microRNAs in pancreatic ductal adenocarcinoma and the functional effect of miR-21 inhibition finds it could be a predictor of disease progression and a possible therapeutic target in part by upregulating PDCD4 in pancreatIC cancer.
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HypoxamiRs and cancer: from biology to targeted therapy.
TL;DR: In this paper, the authors found that increased expression of miR-210, the prototypical hypoxamiR, is associated with adverse prognosis in many tumor types, suggesting that their down-regulation occurs in vivo and contributes to tumor growth.
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A panel of four decreased serum microRNAs as a novel biomarker for early Parkinson's disease
Hui Dong,Cheng Wang,Sunbin Lu,Cui-Yu Yu,Lei Huang,Wu-Ruo Feng,Hui Xu,Xi Chen,Ke Zen,Qiao Yan,Weiguo Liu,Chunni Zhang,Chen-Yu Zhang +12 more
TL;DR: The four serum miRNAs may represent novel biomarkers for the early detection of PD and enabled the differentiation of HY stage 1 and 2 PD patients from controls.
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Identification of miRNA-103 in the cellular fraction of human peripheral blood as a potential biomarker for malignant mesothelioma--a pilot study.
TL;DR: The results of this pilot study show that miR-103 is characterized by a promising sensitivity and specificity and might be a potential minimally-invasive biomarker for the diagnosis of mesothelioma, and support the concept of using the cellular fraction of human blood for biomarker discovery.
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A microRNA expression signature of human solid tumors defines cancer gene targets
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