MicroRNAs in plasma of pancreatic ductal adenocarcinoma patients as novel blood-based biomarkers of disease
Jin Wang,Jinyun Chen,Ping Chang,Aimee Leblanc,Donghui Li,James L. Abbruzzesse,Marsha L. Frazier,Ann M. Killary,Subrata Sen +8 more
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TLDR
Observations show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the future.Abstract:
Development of minimally invasive biomarker assays for early detection and effective clinical management of pancreatic cancer is urgently needed to reduce high morbidity and mortality associated with this malignancy We hypothesized that if aberrantly expressing microRNAs (miRNA) in pancreatic adenocarcinoma tissues are detected in blood plasma, then plasma profiling of these miRNAs might serve as a minimally invasive early detection biomarker assay for this malignancy By using a modified protocol to isolate and quantify plasma miRNAs from heparin-treated blood, we show that miRNA profiling in plasma can differentiate pancreatic adenocarcinoma patients from healthy controls We have profiled four miRNAs, miR-21, miR-210, miR-155, and miR-196a, all implicated in the development of pancreatic cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways Of these, miR-155 has recently been identified as a candidate biomarker of early pancreatic neoplasia, whereas elevated expression of miR196a has been shown to parallel progression of disease The results revealed a sensitivity of 64% and a specificity of 89% with the analyses of plasma levels for this panel of four miRNAs The area under the receiver operating characteristic curve were estimated at 082 and 078 without and with leave-one-out cross-validation scheme, respectively These observations, although a "proof of principle" finding at this time, show the feasibility of developing plasma miRNA profiling as a sensitive and specific blood-based biomarker assay for pancreatic cancer that has the potential of translation to the clinic with additional improvements in the futureread more
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MiRNA predictors of pancreatic cancer chemotherapeutic response: A systematic review and meta-analysis
Madhav Madurantakam Royam,Rithika Ramesh,Ritika Shanker,Shanthi Sabarimurugan,Chellan Kumarasamy,Nachimuthu Ramesh,Kodiveri Muthukalianan Gothandam,Siddharta Baxi,Ajay Gupta,Sunil Krishnan,Rama Jayaraj +10 more
TL;DR: The results highlight multiple miRNAs that have possible associations with modulation of chemotherapy response in pancreatic cancer patients and further studies are needed to discover the molecular mechanisms underlying these associations before they can be suggested for use as biomarkers of response to chemotherapeutic interventions in Pancic cancer.
Journal ArticleDOI
Aggregation-Induced Electrochemiluminescence of the Dichlorobis(1,10-phenanthroline)ruthenium(II) (Ru(phen)2Cl2)/Tri-n-propylamine (TPrA) System in H2O-MeCN Mixtures for Identification of Nucleic Acids.
TL;DR: The abovementioned AIECL behavior was found to be very sensitive to the types and sequences of nucleic acids present in the solution, which provided an effective and novel strategy for distinguishing RNA from DNA and for differentiating different miRNAs.
Journal ArticleDOI
MicroRNA-155 regulates T cell proliferation through targeting GSK3β in cardiac allograft rejection in a murine transplantation model.
TL;DR: The present study provides a better understanding of the pathophysiological process underlying cardiac AR progression and confirms that the miR-155 in LFB can distinguish recipients with AR from syngeneic controls from POD 3 and later.
Journal ArticleDOI
Aberrant microRNAs expression patterns in pancreatic cancer and their clinical translation.
TL;DR: A possible role for miRNAs in the early diagnosis of PC and whether expression levels of specific miRNAAs differed between PC patients, pancreatitis patients, and healthy individuals is investigated.
Journal ArticleDOI
Hypoxamirs and mitochondrial metabolism.
TL;DR: The use of antisense inhibitors is currently being considered in diseases in which hypoxia and metabolic dysregulation predominate, and exploration of pleiotripic miRNA functions will likely continue to offer unique insights into the mechanistic relationships of their downstream target pathways and associated hypoxic phenotypes.
References
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Analyzing real-time PCR data by the comparative C(T) method.
TL;DR: This protocol provides an overview of the comparative CT method for quantitative gene expression studies and various examples to present quantitative gene Expression data using this method.
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Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
TL;DR: It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.
Journal ArticleDOI
A microRNA expression signature of human solid tumors defines cancer gene targets
Stefano Volinia,George A. Calin,Chang Gong Liu,Stefan Ambs,Amelia Cimmino,Fabio Petrocca,Rosa Visone,Marilena V. Iorio,Claudia Roldo,Manuela Ferracin,Robyn L. Prueitt,Nozumu Yanaihara,Giovanni Lanza,Aldo Scarpa,Andrea Vecchione,Massimo Negrini,Curtis C. Harris,Carlo M. Croce +17 more
TL;DR: The results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.
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MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells.
TL;DR: It is shown that the highly malignant human brain tumor, glioblastoma, strongly over-expresses a specific miRNA, miR-21, which may contribute to the malignant phenotype by blocking expression of critical apoptosis-related genes.
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