Molecular architecture of the SARS-CoV-2 virus
Hangping Yao,Yutong Song,Yong Chen,Nanping Wu,Jialu Xu,Chujie Sun,Jiaxing Zhang,Tianhao Weng,Zheyuan Zhang,Zhigang Wu,Linfang Cheng,Danrong Shi,Xiangyun Lu,Jianlin Lei,Max Crispin,Yigong Shi,Lanjuan Li,Sai Li +17 more
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TLDR
Overall, these characterizations have revealed the architecture of the SARS-CoV-2 virus in unprecedented detail, and shed lights on how the virus packs its ∼30 kb long single-segmented RNA in the ∼80 nm diameter lumen.Abstract:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins and the complexes of the spike (S) proteins with the cellular receptor ACE2 or neutralizing antibodies, detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryo-electron tomography (cryo-ET) and subtomogram averaging (STA). Native structures of the S proteins in both pre- and postfusion conformations were determined to average resolutions of 9-11 A. Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed highly similar overall processing states of the native glycans to that of the recombinant glycoprotein glycans. The in situ architecture of the ribonucleoproteins (RNP) and its higher-order assemblies were revealed. These characterizations have revealed the architecture of the SARS-CoV-2 virus to an unprecedented resolution, and shed lights on how the virus packs its ~30 Kb long single-segmented RNA in the ~80 nm diameter lumen. Overall, the results unveiled the molecular architecture and assembly of the SARS-CoV-2 in native context.read more
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SARS-COV-2 Variants: Differences and Potential of Immune Evasion
Sandro M. Hirabara,Tamires Duarte Afonso Serdan,Renata Gorjão,Laureane Nunes Masi,Tania Cristina Pithon-Curi,Dimas Tadeu Covas,Rui Curi,Edison Luiz Durigon +7 more
TL;DR: This review addressed the following critical points concerning VOCs: characteristics of the SARS-CoV-2 V OCs with mutations in the S gene, possible evasion of variants from neutralizing antibodies generated through vaccination, previous infection, or immune therapies, potential risk of new pandemic waves induced by the variants worldwide, and perspectives for further studies and actions.
Journal ArticleDOI
Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles.
Maolin Lu,Pradeep D. Uchil,Wenwei Li,Desheng Zheng,Daniel S. Terry,Jason Gorman,Wei Shi,Baoshan Zhang,Tongqing Zhou,Shilei Ding,Romain Gasser,Jérémie Prévost,Guillaume Beaudoin-Bussières,Sai Priya Anand,Sai Priya Anand,Annemarie Laumaea,Jonathan R. Grover,Lihong Liu,David D. Ho,John R. Mascola,Andrés Finzi,Andrés Finzi,Peter D. Kwong,Scott C. Blanchard,Walther Mothes +24 more
TL;DR: Conformational preferences observed upon expsoure to convalescent plasma or antibodies suggest mechanisms of neutralization involving either competition with hACE2 for binding to the receptor-binding domain (RBD) or allosteric interference with conformational changes required for entry.
Journal ArticleDOI
Advances in COVID-19 mRNA vaccine development
Enyue Fang,Xiaohui Liu,Miao Li,Zelun Zhang,Lifang Song,Baiyu Zhu,Xiao-hong Wu,Jingjing Liu,Danhua Zhao,Yuhua Li +9 more
TL;DR: In this article , the authors summarized current knowledge on the structural characteristics, antigen design strategies, delivery systems, industrialization potential, quality control, latest clinical trials and real-world data of COVID-19 mRNA vaccines as well as mRNA technology.
Posted ContentDOI
The SARS-CoV-2 Nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein.
Shan Lu,Qiaozhen Ye,Digvijay Singh,Elizabeth Villa,Don W. Cleveland,Kevin D. Corbett,Kevin D. Corbett +6 more
TL;DR: It is demonstrated that a centrally located 40 amino acid intrinsically disordered domain drives phase separation of N protein when bound to RNA, with the morphology of the resulting condensates affected by inclusion in the RNA of the putative SARS-CoV-2 packaging signal.
Journal ArticleDOI
A Multiscale Coarse-grained Model of the SARS-CoV-2 Virion.
Alvin Yu,Alexander J. Pak,Peng He,Viviana Monje-Galvan,Lorenzo Casalino,Zied Gaieb,Abigail C. Dommer,Rommie E. Amaro,Gregory A. Voth +8 more
TL;DR: The current status and on-going development of a largely “bottom-up” coarse-grained (CG) model of the SARS-CoV-2 virion is reported and how CG molecular interactions can be derived from all-atom simulations are described.
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