Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis.
Kuei Yang Hsiao,Ya-Chi Lin,Sachin Kumar Gupta,Ning Chang,Laising Yen,H. Sunny Sun,Shaw Jenq Tsai +6 more
TLDR
In this paper, the functional role of a novel circRNA, circCCDC66, in colorectal cancer (CRC) was characterized using RNA-Seq data from matched normal and tumor colon tissue samples.Abstract:
Circular RNA (circRNA) is a class of non-coding RNA whose functions remain mostly unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but direct evidence is scarce. Here we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer (CRC). RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-of-function studies in CRC cell-lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis.read more
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The biogenesis, biology and characterization of circular RNAs.
Lasse Sommer Kristensen,Maria S. Andersen,Lotte V. W. Stagsted,Karoline K. Ebbesen,Thomas B. Hansen,Jørgen Kjems +5 more
TL;DR: Advances in high-throughput RNA sequencing and circRNA-specific computational tools have driven the development of state-of-the-art approaches for their identification, and novel approaches to functional characterization are emerging.
Journal ArticleDOI
Circular RNAs in cancer: opportunities and challenges in the field.
TL;DR: The current knowledge on circRNAs is reviewed in relation to their implications in tumorigenesis as well as their potential as diagnostic and prognostic biomarkers and as possible therapeutic targets in future personalized medicine.
Journal ArticleDOI
The Role of Non-coding RNAs in Oncology
TL;DR: For decades, research into cancer biology focused on the involvement of protein-coding genes, but an explosion of studies into ncRNA biology has shown that they represent a diverse and prevalent group of RNAs, including both oncogenic molecules and those that work in a tumor suppressive manner.
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Noncoding RNA:RNA Regulatory Networks in Cancer.
Jia Jia Chan,Yvonne Tay +1 more
TL;DR: Recent integrative analyses have provided evidence that new computational platforms and experimental approaches can be harnessed together to distinguish key ceRNA interactions in specific cancers, which could facilitate the identification of robust biomarkers and therapeutic targets, and hence, more effective cancer therapies and better patient outcome and survival.
Journal ArticleDOI
Circular RNAs function as ceRNAs to regulate and control human cancer progression.
Yaxian Zhong,Yaxian Zhong,Yajun Du,Yajun Du,Xue Yang,Xue Yang,Yongzhen Mo,Yongzhen Mo,Chunmei Fan,Fang Xiong,Daixi Ren,Daixi Ren,Xin Ye,Xin Ye,Chunwei Li,Chunwei Li,Yumin Wang,Yumin Wang,Fang Wei,Can Guo,Xu Wu,Xiaoling Li,Xiaoling Li,Yong Li,Yong Li,Guiyuan Li,Guiyuan Li,Zhaoyang Zeng,Zhaoyang Zeng,Wei Xiong,Wei Xiong +30 more
TL;DR: The progress in studying the role of circRNAs as ceRNAs in tumors is reviewed and the participation of circ RNAs in signal transduction pathways to regulate cellular functions is highlighted.
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