Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TLDR
Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.Abstract:
The development of nonviral vectors for safe and efficient gene delivery has been gaining considerable attention recently. An ideal nonviral vector must protect the gene against degradation by nuclease in the extracellular matrix, internalize the plasma membrane, escape from the endosomal compartment, unpackage the gene at some point and have no detrimental effects. In comparison to viruses, nonviral vectors are relatively easy to synthesize, less immunogenic, low in cost, and have no limitation in the size of a gene that can be delivered. Significant progress has been made in the basic science and applications of various nonviral gene delivery vectors; however, the majority of nonviral approaches are still inefficient and often toxic. To this end, two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells. PLG nanoparticles were optimized for gene delivery by varying particle surface chemistry using different coating materials that adsorb to the particle surface during formation. A variety of cationic coating materials were studied and compared to more conventional surfactants used for PLG nanoparticle fabrication. Nanoparticles (~200 nm) efficiently encapsulated plasmids encoding for luciferase (80-90%) and slowly released the same for two weeks. After a delay, moderate levels of gene expression appeared at day 5 for certain positively charged PLG particles and gene expression was maintained for at least two weeks. In contrast, gene expression mediated by polyethyleneimine (PEI) ended at day 5. PLG particles were also significantly lessread more
Citations
More filters
Journal ArticleDOI
Non-viral vectors for gene-based therapy
Hao Yin,Rosemary Lynn Kanasty,Ahmed A. Eltoukhy,Arturo J. Vegas,J. Robert Dorkin,Daniel G. Anderson +5 more
TL;DR: The biological barriers to gene delivery in vivo are introduced and recent advances in material sciences, nanotechnology and nucleic acid chemistry that have yielded promising non-viral delivery systems are discussed, some of which are currently undergoing testing in clinical trials.
Journal ArticleDOI
Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology.
Kim E. Sapsford,W. Russ Algar,Lorenzo Berti,Kelly Boeneman Gemmill,Brendan J. Casey,Eunkeu Oh,Michael H. Stewart,Igor L. Medintz +7 more
TL;DR: Chemistries that Facilitate Nanotechnology Kim E. Sapsford,† W. Russ Algar, Lorenzo Berti, Kelly Boeneman Gemmill,‡ Brendan J. Casey,† Eunkeu Oh, Michael H. Stewart, and Igor L. Medintz .
Journal ArticleDOI
Nanochemistry and Nanomedicine for Nanoparticle-based Diagnostics and Therapy
TL;DR: This work presents a new generation of high-performance liquid chromatography platforms for selective separation of Na6(CO3) from Na4(SO4) through Na2SO4 and shows real-world applications in drug discovery and treatment of central nervous system disorders.
Journal ArticleDOI
Stimuli-responsive polymeric nanocarriers for the controlled transport of active compounds: Concepts and applications ☆
TL;DR: This review gives a brief overview about some types of stimuli-responsive nanocarriers with the main focus on organic polymer-based systems.
Journal ArticleDOI
Mesoporous Silica Nanoparticles for Intracellular Controlled Drug Delivery
TL;DR: The latest research on the pathways of entry into live mammalian and plant cells together with intracellular trafficking are described, and the current research progress on the biocompatibility of this material in vitro and in vivo is discussed.
References
More filters
Journal ArticleDOI
Polypropylenimine dendrimer-induced gene expression changes: the effect of complexation with DNA, dendrimer generation and cell type.
TL;DR: Data show for the first time that PPI-dendrimers, separate from their capability as transfection reagents, can intrinsically alter the expression of many endogenous genes that could potentially lead to them exerting multiple biological effects in cells.
Journal ArticleDOI
The NH2 terminus of influenza virus hemagglutinin-2 subunit peptides enhances the antitumor potency of polyarginine-mediated p53 protein transduction.
Hiroyuki Michiue,Kazuhito Tomizawa,Fan Yan Wei,Masayuki Matsushita,Yun Fei Lu,Tomotsugu Ichikawa,Takashi Tamiya,Isao Date,Hideki Matsui +8 more
TL;DR: Protein transduction therapy using polyarginine and HA2 may be useful as a method for cancer therapy because of the ability to efficiently translocate into the nucleus of glioma cells and induce p21WAF1 transcriptional activity more effectively.
Journal ArticleDOI
Stabilisation by freeze-drying of cationically modified silica nanoparticles for gene delivery.
Mohammad Sameti,G. Bohr,M. N. V. Ravi Kumar,Carsten Kneuer,Udo Bakowsky,Manfred Nacken,Helmut Schmidt,Claus-Michael Lehr +7 more
TL;DR: Of the various LPAs screened in the present investigations, trehalose and glycerol were found to be well suited for conservation of cationically modified silica nanoparticles with simultaneous preservation of their DNA-binding and transfection activity in Cos-1 cells.
Journal ArticleDOI
Structure-function correlation of chloroquine and analogues as transgene expression enhancers in nonviral gene delivery.
Jianjun Cheng,Ryan K. Zeidan,Swaroop Mishra,Swaroop Mishra,Aijie Liu,Suzie H. Pun,Rajan P. Kulkarni,Gregory S. Jensen,Nathalie C. Bellocq,Mark E. Davis +9 more
TL;DR: There are at least three mechanistic features of CQ that lead to the enhancement in gene expression: pH buffering in endocytic vesicles, displacement of polycations from the nucleic acids in polyplexes, and alteration of the biophysical properties of the released nucleic acid.
Journal ArticleDOI
Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference
Léa Desigaux,Matthieu Sainlos,Olivier Lambert,Raphaël Chèvre,Emilie Letrou-Bonneval,Jean-Pierre Vigneron,Pierre Lehn,Jean-Marie Lehn,Bruno Pitard +8 more
TL;DR: Cryo-transmission electron microscopy and x-ray-scattering experiments showed that lipidic aminoglycoside derivatives constitute a versatile class of siRNA nanocarriers allowing efficient gene silencing.