Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TLDR
Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.Abstract:
The development of nonviral vectors for safe and efficient gene delivery has been gaining considerable attention recently. An ideal nonviral vector must protect the gene against degradation by nuclease in the extracellular matrix, internalize the plasma membrane, escape from the endosomal compartment, unpackage the gene at some point and have no detrimental effects. In comparison to viruses, nonviral vectors are relatively easy to synthesize, less immunogenic, low in cost, and have no limitation in the size of a gene that can be delivered. Significant progress has been made in the basic science and applications of various nonviral gene delivery vectors; however, the majority of nonviral approaches are still inefficient and often toxic. To this end, two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells. PLG nanoparticles were optimized for gene delivery by varying particle surface chemistry using different coating materials that adsorb to the particle surface during formation. A variety of cationic coating materials were studied and compared to more conventional surfactants used for PLG nanoparticle fabrication. Nanoparticles (~200 nm) efficiently encapsulated plasmids encoding for luciferase (80-90%) and slowly released the same for two weeks. After a delay, moderate levels of gene expression appeared at day 5 for certain positively charged PLG particles and gene expression was maintained for at least two weeks. In contrast, gene expression mediated by polyethyleneimine (PEI) ended at day 5. PLG particles were also significantly lessread more
Citations
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Journal ArticleDOI
Non-viral vectors for gene-based therapy
Hao Yin,Rosemary Lynn Kanasty,Ahmed A. Eltoukhy,Arturo J. Vegas,J. Robert Dorkin,Daniel G. Anderson +5 more
TL;DR: The biological barriers to gene delivery in vivo are introduced and recent advances in material sciences, nanotechnology and nucleic acid chemistry that have yielded promising non-viral delivery systems are discussed, some of which are currently undergoing testing in clinical trials.
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Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology.
Kim E. Sapsford,W. Russ Algar,Lorenzo Berti,Kelly Boeneman Gemmill,Brendan J. Casey,Eunkeu Oh,Michael H. Stewart,Igor L. Medintz +7 more
TL;DR: Chemistries that Facilitate Nanotechnology Kim E. Sapsford,† W. Russ Algar, Lorenzo Berti, Kelly Boeneman Gemmill,‡ Brendan J. Casey,† Eunkeu Oh, Michael H. Stewart, and Igor L. Medintz .
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Nanochemistry and Nanomedicine for Nanoparticle-based Diagnostics and Therapy
TL;DR: This work presents a new generation of high-performance liquid chromatography platforms for selective separation of Na6(CO3) from Na4(SO4) through Na2SO4 and shows real-world applications in drug discovery and treatment of central nervous system disorders.
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Stimuli-responsive polymeric nanocarriers for the controlled transport of active compounds: Concepts and applications ☆
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Mesoporous Silica Nanoparticles for Intracellular Controlled Drug Delivery
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References
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Colin W. Pouton,Len Seymour +1 more
TL;DR: Present knowledge of the biodistribution and cellular interactions of gene delivery systems are summarized and how improvements in gene delivery will be accomplished in the future are considered.
Journal ArticleDOI
In Vivo Delivery of a Bcl-xL Fusion Protein Containing the TAT Protein Transduction Domain Protects against Ischemic Brain Injury and Neuronal Apoptosis
Guodong Cao,Wei Pei,Hailiang Ge,Qinhua Liang,Yumin Luo,Frank R. Sharp,Aigang Lu,Ruiqiong Ran,Steven H. Graham,Steven H. Graham,Jun Chen,Jun Chen +11 more
TL;DR: The creation of a PTD fusion protein, designated as PTD-HA-Bcl-xL, which contains the protein transduction domain (PTD) derived from the human immunodeficiency TAT protein is demonstrated, which is highly efficient in transducing into primary neurons in cultures and potently inhibited staurosporin-induced neuronal apoptosis.
Journal ArticleDOI
Antennapedia and HIV Transactivator of Transcription (TAT) “Protein Transduction Domains” Promote Endocytosis of High Molecular Weight Cargo upon Binding to Cell Surface Glycosaminoglycans
Sandra Console,Cornelia Marty,Carlos Garcia-Echeverria,Reto A. Schwendener,Kurt Ballmer-Hofer +4 more
TL;DR: The data reported here support the idea that certain PTDs promote cellular uptake via endocytosis and require the expression of negatively charged glycosaminoglycans on the surface of the target cells, and support the view that PTD-mediated cellular uptake is energy-independent.
Journal ArticleDOI
Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells.
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