Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
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Fingolimod Modulates the Gene Expression of Proteins Engaged in Inflammation and Amyloid-Beta Metabolism and Improves Exploratory and Anxiety-Like Behavior in Obese Mice.
Przemysław L. Wencel,Kamilla Blecharz-Klin,Agnieszka Piechal,Justyna Pyrzanowska,Dagmara Mirowska-Guzel,Robert P. Strosznajder +5 more
Journal ArticleDOI
Effects of exercise intensity on spatial memory performance and hippocampal synaptic function in SAMP8 mice
TL;DR: In this paper , the authors showed that eight weeks of treadmill exercise beginning in four-month old mice improved spatial memory and recognition memory in six-month-old SAMP8 mice, while the Con group exhibited impaired spatial memory, recognition memory, and hippocampal neuron morphology.
Journal ArticleDOI
Inhibition of carnitine palmitoyl-transferase 1 is a potential target in a mouse model of Parkinson’s disease
Michael Sloth Trabjerg,Dennis Christian Andersen,Pam Huntjens,Kasper Mørk,Nikolaj Warming,Ulla Bismark Kullab,Marie Louise Nibelius Skjønnemand,Michal K. Oklinski,Kirsten Egelund Oklinski,Luise Bolther,Lona J. Kroese,Colin Pritchard,Ivo J. Huijbers,Angelique Corthals,Mads Toft Søndergaard,Henrik Kjeldal,Cecilie Fjord Morre Pedersen,John Nieland +17 more
TL;DR: In this article , the effect of downregulating CPT1, either genetically with a Cpt1a P479L mutation or medicinally on Parkinson's disease (PD) using chronic rotenone mouse models using C57Bl/6J and Park2 knockout mice.
Posted ContentDOI
microRNA-138 controls hippocampal interneuron function and short-term memory
Daswani R,Gilardi C,Michael Soutschek,Weiss K,Bicker S,Fiore R,Christoph Dieterich,Pierre-Luc Germain,Winterer J,Gerhard Schratt +9 more
TL;DR: This article showed that miR-138-5p regulates the expression of presynaptic genes in hippocampal parvalbumin-expressing inhibitory interneurons to control short-term memory.
Posted ContentDOI
Lighter compared to deeper isoflurane anaesthesia can affect recognition memory in male C57BL/6 mice
TL;DR: The results raise awareness of the need for careful consideration of the depth of anaesthesia used, especially the use of light isoflurane anaesthesia, which is often chosen to provide animal immobilization during non-invasive procedures.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.