Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
Citations
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Journal ArticleDOI
Novel Object Recognition Test for the Investigation of Learning and Memory in Mice.
TL;DR: The object recognition test is a relatively low-stress, efficient test for memory in mice, and is appropriate for the detection of neuropsychological changes following pharmacological, biological, or genetic manipulations.
Journal ArticleDOI
Hypothalamic stem cells control ageing speed partly through exosomal miRNAs
Yalin Zhang,Min Soo Kim,Baosen Jia,Jingqi Yan,Juan Pablo Zuniga-Hertz,Cheng Han,Dongsheng Cai +6 more
TL;DR: Development of several mouse models in which hypothalamic stem/progenitor cells that co-express Sox2 and Bmi1 are ablated shows that ageing in mice started with a substantial loss of these hypothalamic cells, and ageing speed is substantially controlled by hypothalamicstem cells, partially through the release of exosomal miRNAs.
Journal ArticleDOI
Multi-sensory Gamma Stimulation Ameliorates Alzheimer’s-Associated Pathology and Improves Cognition
Anthony J Martorell,Anthony J Martorell,Abigail L. Paulson,Ho-Jun Suk,Ho-Jun Suk,Fatema Abdurrob,Fatema Abdurrob,Gabrielle T. Drummond,Gabrielle T. Drummond,Webster Guan,Jennie Z. Young,Jennie Z. Young,David Nam Woo Kim,David Nam Woo Kim,Oleg Kritskiy,Oleg Kritskiy,Scarlett J. Barker,Scarlett J. Barker,Vamsi Mangena,Stephanie M. Prince,Emery N. Brown,Kwanghun Chung,Edward S. Boyden,Edward S. Boyden,Annabelle C. Singer,Li-Huei Tsai,Li-Huei Tsai,Li-Huei Tsai +27 more
TL;DR: Auditory tone stimulation that drove gamma frequency neural activity in auditory cortex (AC) and hippocampal CA1 improved spatial and recognition memory and reduced amyloid in AC and hippocampus of 5XFAD mice, suggesting GENUS can be achieved through multiple sensory modalities with wide-ranging effects across multiple brain areas to improve cognitive function.
Journal ArticleDOI
A Ketogenic Diet Extends Longevity and Healthspan in Adult Mice.
Megan N. Roberts,Marita A. Wallace,Alexey Tomilov,Zeyu Zhou,George R. Marcotte,Dianna Tran,Gabriella Perez,Elena Gutiérrez-Casado,Shinichiro Koike,Trina A. Knotts,Denise M. Imai,Stephen M Griffey,Kyoungmi Kim,Kevork Hagopian,Marissa Z. McMackin,Fawaz G. Haj,Keith Baar,Gino A Cortopassi,Jon J. Ramsey,Jose Alberto Lopez-Dominguez +19 more
TL;DR: It is demonstrated that a KD extends longevity and healthspan in mice and regulated mTORC1 signaling in a tissue-dependent manner.
Journal ArticleDOI
Microbiota modulation counteracts Alzheimer's disease progression influencing neuronal proteolysis and gut hormones plasma levels.
Laura Bonfili,Valentina Cecarini,Sara Berardi,Silvia Scarpona,Jan S. Suchodolski,Cinzia Nasuti,Dennis Fiorini,Maria Chiara Boarelli,Giacomo Rossi,Anna Maria Eleuteri +9 more
TL;DR: It is shown that modulation of the microbiota induces positive effects on neuronal pathways that are able to slow down the progression of Alzheimer’s disease.
References
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Journal ArticleDOI
Synergistic effect of acetylcholinesterase inhibition (donepezil) and 5-HT(4) receptor activation (RS67333) on object recognition in mice.
Thomas Freret,Valentine Bouet,Anne Quiedeville,Gerald Nee,Patrick Dallemagne,Christophe Rochais,Michel Boulouard +6 more
TL;DR: Activation of 5-HT(4)R combined with inhibition of AChE with subactive doses of RS67333 and of DNPZ has synergistic effects on memory performances in mice, affording new hope to chemist for the synthesis of MTDL.
Journal ArticleDOI
Environmental enrichment enhances episodic-like memory in association with a modified neuronal activation profile in adult mice.
Marianne Leger,Anne Quiedeville,Eleni Paizanis,Sharuja Natkunarajah,Thomas Freret,Michel Boulouard,Pascale Schumann-Bard +6 more
TL;DR: The results suggest that the persistence of object recognition memory in EC could be related to a reorganization of neuronal networks occurring as early as the memory encoding.
Journal ArticleDOI
Rats' novel object interaction as a measure of environmental familiarity
TL;DR: This paper found that 3min of exposure to the environment was sufficient to increase object interaction above unfamiliar controls even when testing occurred up to 48h after initial exposure to an environment; 1 or 1.5 min of exposure was not sufficient.