Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
Citations
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Low-level laser therapy for beta amyloid toxicity in rat hippocampus.
Yujiao Lu,Ruimin Wang,Ruimin Wang,Yan Dong,Donovan Tucker,Ningjun Zhao,Ejaz Ahmed,Ling Zhu,Timon Cheng-Yi Liu,Robert M. Cohen,Quanguang Zhang +10 more
TL;DR: Low-level laser irradiation could represent a new promising therapeutic strategy for AD by alleviating a broad spectrum of Aβ-induced pathology that includes mitochondrial dysfunction, oxidative stress, neuroinflammation, neuronal apoptosis, and tau pathology.
Journal ArticleDOI
Moderate UV Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain.
Hongying Zhu,Ning Wang,Lei Yao,Qi Chen,Ran Zhang,Junchao Qian,Yiwen Hou,Weiwei Guo,Sijia Fan,Siling Liu,Qiaoyun Zhao,Feng Du,Xin Zuo,Yujun Guo,Yan Xu,Jiali Li,Tian Xue,Kai Zhong,Xiaoyuan Song,Guangming Huang,Wei Xiong,Wei Xiong +21 more
TL;DR: It is shown that moderate UV exposure elevated blood urocanic acid (UCA), which then crossed the blood-brain barrier and revealed a novel intra-neuronal metabolic pathway converting UCA to glutamate (GLU) after UV exposure, which could contribute to some of the sunlight-induced neurobehavioral changes.
Journal ArticleDOI
Increased Microglial Exosomal miR-124-3p Alleviates Neurodegeneration and Improves Cognitive Outcome after rmTBI.
Xintong Ge,Mengtian Guo,Tianpeng Hu,Wenzhu Li,Shan Huang,Zhenyu Yin,Ying Li,Fanglian Chen,Luoyun Zhu,Chunsheng Kang,Rongcai Jiang,Rongcai Jiang,Ping Lei,Jianning Zhang,Jianning Zhang +14 more
TL;DR: In in vitro experiments, microglial exosomes with upregulated miR-124-3p (EXO-124) alleviated neurodegeneration in repetitive scratch-injured neurons and improved the cognitive outcome after rmTBI, suggesting a promising therapeutic strategy for future clinical translation.
Journal ArticleDOI
Distinct and Dynamic ON and OFF Neural Ensembles in the Prefrontal Cortex Code Social Exploration.
Bo Liang,Lifeng Zhang,Giovanni Barbera,Wenting Fang,Jing Zhang,Xiaochun Chen,Rong Chen,Yun Li,Yun Li,Da-Ting Lin,Da-Ting Lin +10 more
TL;DR: This work utilized miniScopes to record calcium activities from hundreds of excitatory neurons in the mPFC while mice freely explored restrained social targets in the absence or presence of the psychedelic drug phencyclidine, and identified distinct and dynamic ON and OFF neural ensembles that displayed opposing activities to code real-time behavioral information.
Journal ArticleDOI
The pathological role of NLRs and AIM2 inflammasome-mediated pyroptosis in damaged blood-brain barrier after traumatic brain injury.
Xintong Ge,Wenzhu Li,Shan Huang,Zhenyu Yin,Xin Xu,Fanglian Chen,Xiaodong Kong,Haichen Wang,Jianning Zhang,Jianning Zhang,Ping Lei +10 more
TL;DR: It is found that TBI led to NLRs and AIM2 inflammasome-mediated pyroptosis in brain microvascular endothelial cells (BMVECs) from injured cerebral cortex, and inhibiting pyroPTosis could alleviate TBI-induced BBB leakage, brain edema, loss of tight junction proteins, and the inflammatory response in injured BMV ECs.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.