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Journal ArticleDOI

Object recognition test in mice

TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.
Abstract
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.

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Citations
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Journal ArticleDOI

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice.

TL;DR: The object recognition test is a relatively low-stress, efficient test for memory in mice, and is appropriate for the detection of neuropsychological changes following pharmacological, biological, or genetic manipulations.
Journal ArticleDOI

Hypothalamic stem cells control ageing speed partly through exosomal miRNAs

TL;DR: Development of several mouse models in which hypothalamic stem/progenitor cells that co-express Sox2 and Bmi1 are ablated shows that ageing in mice started with a substantial loss of these hypothalamic cells, and ageing speed is substantially controlled by hypothalamicstem cells, partially through the release of exosomal miRNAs.
Journal ArticleDOI

Microbiota modulation counteracts Alzheimer's disease progression influencing neuronal proteolysis and gut hormones plasma levels.

TL;DR: It is shown that modulation of the microbiota induces positive effects on neuronal pathways that are able to slow down the progression of Alzheimer’s disease.
References
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Journal ArticleDOI

The role of environmental familiarization in novel-object preference.

TL;DR: The experiments in this report examined the behavioral variables modulating novel-object preference in a widely used 'object recognition' preparation that takes advantage of the tendency of rats to interact more with a novel than a familiar 'sample' object in a free-choice situation.
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Hippocampal neurotransmitter efflux during one-trial novel object recognition in rats

TL;DR: Assessing hippocampal acetylcholine, glutamate and GABA efflux when rats were exposed to familiar objects, and when given the option to explore familiar and novel objects suggests that the hippocampus, and in particular, hippocampal glutamate, may be involved in memory processes during novelty recognition paradigms.
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Continuous enriched environment improves learning and memory in adult NMRI mice through theta burst-related-LTP independent mechanisms but is not efficient in advanced aged animals.

TL;DR: It is indicated that continuous EE is able to improve cognitive abilities in adult NMRI female mice, that does not correlate with changes in theta burst-related synaptic plasticity within neuronal networks.

Short communication Temporal stability of novelty exploration in mice exposed to different open field tests

TL;DR: In this paper, the authors investigated behavioral activity and temporal distribution (patterning) of mouse exploration in different open field (OF) arenas and found that mice exhibited a high degree of similarity in the temporal profile of novelty-induced horizonta l and vertical exploration, which remained stable in subsequent OF exposures.
Journal ArticleDOI

Disrupted allocentric but preserved egocentric spatial learning in transgenic mice with impaired glucocorticoid receptor function.

TL;DR: The data suggest that functional underactivity of GRs at the level of the hippocampus induces a deficit in allocentric navigation while sparing egocentric navigation and object recognition.
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