Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
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Reducing the RNA binding protein TIA1 protects against tau-mediated neurodegeneration in vivo
Daniel J. Apicco,Peter E.A. Ash,Brandon F. Maziuk,Chelsey Jenna LeBlang,Maria Medalla,Ali Al Abdullatif,Antonio Ferragud,Emily Botelho,Heather I. Ballance,Uma Dhawan,Samantha Boudeau,Anna Lourdes Cruz,Daniel Kashy,Aria Wong,Lisa R. Goldberg,Neema Yazdani,Cheng Zhang,Choong Y. Ung,Yorghos Tripodis,Nicholas M. Kanaan,Tsuneya Ikezu,Pietro Cottone,John D. Leszyk,Hu Li,Jennifer I. Luebke,Camron D. Bryant,Benjamin Wolozin +26 more
TL;DR: It is shown that reducing the RBP T-cell intracellular antigen 1 (TIA1) in vivo protects against neurodegeneration and prolongs survival in transgenic P301S Tau mice, and suggests a key role for RNA binding proteins in the pathophysiology of tau.
Journal ArticleDOI
Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
Keisuke Yanagida,Catherine H. Liu,Giuseppe Faraco,Sylvain Galvani,Sylvain Galvani,Helen K. Smith,Nathalie Burg,Josef Anrather,Teresa Sanchez,Costantino Iadecola,Timothy Hla +10 more
TL;DR: The BBB-regulatory role of endothelial sphingosine 1–phosphate (S1P) receptor-1, a G protein-coupled receptor known to promote the barrier function in peripheral vessels, is reported to raise the possibility that endothelial S1P1 inhibition may be a strategy for transient BBB opening and delivery of small molecules into the CNS.
Journal ArticleDOI
Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia
Eduardo Gascon,Kelleen Lynch,Hongyu Ruan,Sandra Almeida,Jamie M. Verheyden,William W. Seeley,Dennis W. Dickson,Leonard Petrucelli,Danqiong Sun,Jian Jiao,Hongru Zhou,Mira Jakovcevski,Schahram Akbarian,Schahram Akbarian,Wei-Dong Yao,Wei-Dong Yao,Fen-Biao Gao +16 more
TL;DR: This work shows that forebrain-specific expression of FTD-associated mutant CHMP2B in mice causes several age-dependent neurodegenerative phenotypes, including social behavioral impairments, and identifies a previously undescribed mechanism involving miR-124 and AMPARs in regulating social behavior in FTD and suggests a potential therapeutic avenue.
Journal ArticleDOI
Brain changes in a maternal immune activation model of neurodevelopmental brain disorders.
Lara Bergdolt,Anna Dunaevsky +1 more
TL;DR: The behavior and brain phenotypes that have been characterized in MIA offspring are reviewed, including the studies that have looked at the interaction between maternal immune activation and genetic risk factors for autism spectrum disorder or schizophrenia.
Journal ArticleDOI
Loss of Adaptive Myelination Contributes to Methotrexate Chemotherapy-Related Cognitive Impairment
Anna Geraghty,Erin M. Gibson,Reem A. Ghanem,Jacob J. Greene,Alfonso Ocampo,Andrea K. Goldstein,Lijun Ni,Tao Yang,Rebecca M. Marton,Sergiu P. Paşca,Michael E. Greenberg,Frank M. Longo,Michelle Monje +12 more
TL;DR: It is demonstrated that remote MTX exposure blocks activity-regulated myelination, a molecular mechanism required for adaptive myelinations that is aberrant in CRCI due to microglial activation.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.