Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
Citations
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Environmental Enrichment Duration Differentially Affects Behavior and Neuroplasticity in Adult Mice
Marianne Leger,Eleni Paizanis,Kwamivi Dzahini,Anne Quiedeville,Valentine Bouet,Jean-Christophe Cassel,Thomas Freret,Pascale Schumann-Bard,Michel Boulouard +8 more
TL;DR: The results suggest that the duration of exposure is a keystone of the beneficial behavioral and neurobiological effects of environmental enrichment.
Journal ArticleDOI
Design of donecopride, a dual serotonin subtype 4 receptor agonist/acetylcholinesterase inhibitor with potential interest for Alzheimer's disease treatment
Cédric Lecoutey,Damien Hedou,Thomas Freret,Patrizia Giannoni,Florence Gaven,Marc Since,Valentine Bouet,Céline Ballandonne,Sophie Corvaisier,Aurélie Malzert Fréon,Serge Mignani,Thierry Cresteil,Michel Boulouard,Sylvie Claeysen,Christophe Rochais,Patrick Dallemagne +15 more
TL;DR: In this article, a multitarget-directed ligand that is able to exert not only a symptomatic but also, a disease-modifying effect against Alzheimer's disease (AD) was proposed.
Journal ArticleDOI
Age-related cognitive impairment is associated with long-term neuroinflammation and oxidative stress in a mouse model of episodic systemic inflammation
Joana C. D’Avila,Luciana D. Siqueira,Aurélien Mazeraud,Estefania P. Azevedo,Debora Foguel,Hugo C. Castro-Faria-Neto,Tarek Sharshar,Fabrice Chrétien,Fernando A. Bozza +8 more
TL;DR: The data show that aged mice have increased susceptibility to episodic systemic inflammation, and indicates that a neuroinflammation and oxidative stress are pathophysiological mechanisms of age-related cognitive impairments.
Journal ArticleDOI
Amelioration of Alzheimer’s disease pathology by mitophagy inducers identified via machine learning and a cross-species workflow
Chenglong Xie,Xuxu Zhuang,Zhangming Niu,Rui-Ting Ai,Sofie Lautrup,Shuangjia Zheng,Yinghui Jiang,Ruiyu Han,Tanima Sen Gupta,Shuqin Cao,Maria Jose Lagartos-Donate,Cui-Zan Cai,Liwei Xie,Domenica Caponio,Wen-Wen Wang,Tomas Schmauck-Medina,Jianying Zhang,He-ling Wang,Guofeng Lou,Xianglu Xiao,Wenhua Zhang,Konstantinos Palikaras,Guang Yang,Kim A. Caldwell,Guy A. Caldwell,Hanyuan Shen,Hilde Nilsen,Jiahua Lu,Evandro Fei Fang +28 more
TL;DR: In this paper , the authors report the combined use of unsupervised machine learning (involving vector representations of molecular structures, pharmacophore fingerprinting and conformer fingerprinting) and a cross-species approach for the screening and experimental validation of new mitophagy-inducing compounds.
Journal ArticleDOI
Neuroinflammation-Associated Aspecific Manipulation of Mouse Predator Fear by Toxoplasma gondii
Madlaina Boillat,Pierre-Mehdi Hammoudi,Sunil Kumar Dogga,Stéphane Pagès,Maged Goubran,Ivan Rodriguez,Dominique Soldati-Favre +6 more
TL;DR: Using a set of complementary behavioral tests, this work provides strong evidence that T. gondii lowers general anxiety in infected mice, increases explorative behaviors, and surprisingly alters predator aversion without selectivity toward felids, and refute the myth of a selective loss of cat fear in T. Gondii-infected mice.
References
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A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.