Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
Citations
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Loss of C/EBPδ Exacerbates Radiation-Induced Cognitive Decline in Aged Mice due to Impaired Oxidative Stress Response
Sudip Banerjee,Tyler Alexander,Debajyoti Majumdar,Thomas Groves,Frederico Kiffer,Jing Wang,Akshita Gorantla,Antiño R. Allen,Snehalata A. Pawar +8 more
TL;DR: It is suggested that Cebpd may protect from IR-induced neurocognitive dysfunction by suppressing oxidative stress in aged mice.
Journal ArticleDOI
Distinct behavioral traits and associated brain regions in mouse models for obsessive-compulsive disorder.
TL;DR: In this paper, the brain regions related to the behavioral heterogeneity of OCD-like behavioral traits are identified. But, the authors did not identify the specific brain regions that are associated with these distinct behavioral traits.
Journal ArticleDOI
PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival
Yonejung Yoon,Georgios Voloudakis,Nathan Doran,Emily Zhang,Christina Dimovasili,Lei Chen,Zhiping Shao,Spyros Darmanis,Cheuk Y. Tang,Jun Tang,Victoria X. Wang,Patrick R. Hof,Nikolaos K. Robakis,Anastasios Georgakopoulos +13 more
TL;DR: Together, the data show that PS1 FAD mutations impede the EphB4/ephrinB2-mediated angiogenic functions of ECs and impair brain neovascularization, neuronal survival and cognitive recovery following ischemia.
Journal ArticleDOI
Early postnatal injections of whole vaccines compared to placebo controls: Differential behavioural outcomes in mice.
Housam Eidi,Janice Yoo,Suresh C. Bairwa,Michael Kuo,Eric C. Sayre,Lucija Tomljenovic,Christopher A. Shaw +6 more
TL;DR: The observed neurodevelopmental alterations did not show a linear relationship with vaccine dose, suggesting that the single dose gave a saturated response, and the outcomes seemed to be sex-dependent and transient with age.
Journal ArticleDOI
BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
TL;DR: BehaviorDEPOT as mentioned in this paper is a simple, flexible software program that can detect behavior from video timeseries and can analyze the results of experimental assays, including fear conditioning, decision-making in a T-maze, open field, elevated plus maze, and novel object exploration.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.