Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
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Human Mesenchymal Stem Cells Prevent Neurological Complications of Radiotherapy.
Bernat Soria,Alejandro Martin-Montalvo,Yolanda Aguilera,Nuria Mellado-Damas,Javier López-Beas,Isabel Herrera-Herrera,Escarlata López,Juan A. Barcia,Manuel Alvarez-Dolado,Abdelkrim Hmadcha,Vivian Capilla-Gonzalez +10 more
TL;DR: It is demonstrated that intranasal administration of human mesenchymal stem cells (hMSCs) promote radiation-induced brain injury repair, improving neurological function, and encourages the therapeutic use of hMSCs as a non-invasive approach to prevent neurological complications of radiotherapy, improving the quality of life of brain tumor patients.
Journal ArticleDOI
Neural blastocyst complementation enables mouse forebrain organogenesis.
Amelia N. Chang,Zhuoyi Liang,Hai-Qiang Dai,Aimee M. Chapdelaine-Williams,Nick Andrews,Roderick T. Bronson,Bjoern Schwer,Frederick W. Alt +7 more
TL;DR: Neural blastocyst complementation creates a vacant forebrain niche in host embryos that can be populated by donor embryonic stem cell-derived dorsal telencephalic progenitors, resulting in a mouse brain organogenesis model and is concluded to be a rapid and efficient approach to generate complex mouse models for studying forebrain functions.
Posted ContentDOI
Regulation of blood-brain barrier integrity and cognition by the microbiome-associated methylamines trimethylamine N-oxide and trimethylamine
Lesley Hoyles,Matthew G. Pontifex,Ildefonso Rodríguez-Ramiro,Ildefonso Rodríguez-Ramiro,M. Areeb Anis-Alavi,Tom Snelling,Egle Solito,Egle Solito,Sonia Fonseca,Ana L. Carvalho,Simon R. Carding,Simon R. Carding,Michael Müller,Robert C. Glen,Robert C. Glen,David Vauzour,Simon McArthur +16 more
TL;DR: In this paper, an integrated in vitro/in vivo approach was used to show that physiologically relevant concentrations of the dietary methylamine trimethylamine N- oxide (TMAO) enhanced and protected blood-brain barrier (BBB) integrity, acting through the tight junction regulator annexin A1.
Journal ArticleDOI
Appropriate exercise level attenuates gut dysbiosis and valeric acid increase to improve neuroplasticity and cognitive function after surgery in mice.
Zhongmeng Lai,Zhongmeng Lai,Weiran Shan,Jun Li,Jia Min,Jia Min,Xianzhang Zeng,Xianzhang Zeng,Zhiyi Zuo +8 more
TL;DR: The authors showed that low-intensity exercise improved learning and memory in surgery mice and attenuated surgery-induced neuroinflammation and decrease of gut microbiota diversity in non-exercise mice.
Journal ArticleDOI
Hypertension impairs hippocampus-related adult neurogenesis, CA1 neuron dendritic arborization and long-term memory
Yao Hsiang Shih,S. F. Tsai,S. H. Huang,Y. T. Chiang,Michael W. Hughes,Shih-Ying Wu,C. W. Lee,T. T. Yang,Yu Min Kuo +8 more
TL;DR: Hypertension impairs hippocampus-associated long-term memory, dendritic arborization and neurogenesis, which may be caused by down-regulation of brain-derived neurotrophic factor signaling pathways.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.