Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
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Image-guided phenotyping of ovariectomized mice: altered functional connectivity, cognition, myelination, and dopaminergic functionality
Cynthia Anckaerts,Jaana van Gastel,Valerie Leysen,Rukun Hinz,Abdelkrim Azmi,Pascal Simoens,Disha Shah,Firat Kara,A. Langbeen,Peter E.J. Bols,Charlotte Laloux,Vincent Prevot,Marleen Verhoye,Stuart Maudsley,Annemie Van der Linden +14 more
TL;DR: It is shown using resting-state functional magnetic resonance imaging that ovariectomy and consequent hypothalamus-pituitary-gonadal disruption result in the selective dysconnectivity of 2 discrete brain regions in mice, which coincided with cognitive deficits and an underlying pathological molecular phenotype involving an imbalance of neurodevelopmental/neurodegenerative signaling.
Journal ArticleDOI
Neutral Sphingomyelinase is an Affective Valence-Dependent Regulator of Learning and Memory.
Liubov S. Kalinichenko,Laila Abdel-Hafiz,An-Li Wang,Christiane Mühle,Nadine Rösel,Fabian Schumacher,Fabian Schumacher,Burkhard Kleuser,Irena Smaga,Małgorzata Frankowska,Małgorzata Filip,Gerd Schaller,Tanja Richter-Schmidinger,Bernd Lenz,Bernd Lenz,Erich Gulbins,Erich Gulbins,Johannes Kornhuber,André W C Oliveira,Marilia Barros,Marilia Barros,Joseph P. Huston,Christian P. Müller +22 more
TL;DR: A positive association of serum NSM activity with long-term appetitively motivated memory in nonhuman primates and in healthy humans is confirmed and suggests a new sphingolipid mechanism of de-novo learning and memory, which is based onNSM activity.
Journal ArticleDOI
Extracellular vesicles mediate the communication of adipose tissue with brain and promote cognitive impairment associated with insulin resistance.
TL;DR: In this paper , the authors demonstrated that adipose tissue-derived extracellular vesicles (EVs) and their cargo microRNAs (miRNAs) mediate inter-organ communication between adipose tissues and the brain, which can be transferred into the brain in a membrane protein-dependent manner.
Journal ArticleDOI
Treatment with scFv-h3D6 Prevented Neuronal Loss and Improved Spatial Memory in Young 3xTg-AD Mice by Reducing the Intracellular Amyloid-β Burden.
Gisela Esquerda-Canals,Alejandro R Roda,Joaquim Martí-Clúa,Laia Montoliu-Gaya,Laia Montoliu-Gaya,Geovanny Rivera-Hernández,Sandra Villegas +6 more
TL;DR: Findings indicated that the mechanism underlying the therapeutic effect of scFv-h3D6 was the clearance of intracellular Aβ, with subsequent prevention of neuronal loss and amelioration of cognitive disabilities.
Journal ArticleDOI
Behavioral and neurochemical characterization of the mlh mutant mice lacking otoconia.
Marianna Manes,Mariana de Souza Aranha Garcia-Gomes,Thaísa Meira Sandini,Julia Zaccarelli-Magalhães,Jorge Camilo Flório,Sandra Regina Alexandre-Ribeiro,Danilo Wadt,Maria Martha Bernardi,Silvia Maria Gomes Massironi,Claudia Madalena Cabrera Mori +9 more
TL;DR: In the mlh mutant mice, otoconia deficiency reduced the motor, sensory and spatial learning behaviors likely by impairing balance and the role of the dopaminergic system was ruled out.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.