Journal ArticleDOI
Object recognition test in mice
Marianne Leger,Anne Quiedeville,Valentine Bouet,Benoit Haelewyn,Michel Boulouard,Pascale Schumann-Bard,Thomas Freret +6 more
TLDR
This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session, and describes the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases.Abstract:
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.read more
Citations
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In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy
Gaia Colasante,Yichen Qiu,Luca Massimino,Claudia Di Berardino,Jonathan H Cornford,Albert Snowball,Mikail Weston,Steffan P Jones,Serena Giannelli,Andreas Lieb,Stephanie Schorge,Stephanie Schorge,Dimitri M. Kullmann,Vania Broccoli,Gabriele Lignani +14 more
TL;DR: In this article, the authors applied a doxycycline-inducible CRISPRa technology to increase the expression of the potassium channel gene Kcna1 (encoding Kv1.1) in mouse hippocampal excitatory neurons.
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Light modulates hippocampal function and spatial learning in a diurnal rodent species: A study using male nile grass rat (Arvicanthis niloticus).
TL;DR: The results suggest that lighting conditions influence cognitive function of grass rats in a way similar to that observed in humans, such that bright light is beneficial over dim light for cognitive performance.
Journal ArticleDOI
A Review of Freely Available, Open-Source Software for the Automated Analysis of the Behavior of Adult Zebrafish.
TL;DR: Freely available software for the automated analysis of behavior in animal models such as adult zebrafish is shown and information is provided for researchers and students looking for quick, easy-to-implement, and inexpensive behavior analysis alternatives.
Journal ArticleDOI
Learning and memory are impaired in the object recognition task during metestrus/diestrus and after sleep deprivation.
Joshua Cordeira,Sai Saroja Kolluru,Heather Rosenblatt,Jenny Kry,Robert E. Strecker,Robert W. McCarley +5 more
TL;DR: The results show that sleep deprived mice in proestrus/estrus had learning deficits and memory impairments, like mice in metestris/diestrus, however, results did not support the hypothesis that sleep deprivation impacts learning and memory in female mice by interfering with the estrous cycle.
Journal ArticleDOI
Docosahexaenoic acid modulates brain-derived neurotrophic factor via GPR40 in the brain and alleviates diabesity-associated learning and memory deficits in mice.
Chandan Sona,Ajeet Kumar,Shalini Dogra,Boda Arun Kumar,Deepmala Umrao,Prem N. Yadav,Prem N. Yadav +6 more
TL;DR: Evidence is provided that DHA stimulation of GPR40 mediate some of DHA's beneficial effects in metabolic syndrome and identify G PR40 as a viable therapeutic target for the treatment of CNS-related comorbidities associated with diabesity.
References
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Journal ArticleDOI
A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data.
TL;DR: A new memory test in rats, based on the differential exploration of familiar and new objects, which is comparable to memory tests currently used in man and allows interspecies comparisons.
Journal ArticleDOI
The use of the elevated plus maze as an assay of anxiety-related behavior in rodents
Alicia A. Walf,Cheryl A. Frye +1 more
TL;DR: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior.
Journal ArticleDOI
Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'
Rick A. Bevins,Joyce Besheer +1 more
TL;DR: The object-recognition task has been used to study mutant mice, aging deficits, early developmental influences, nootropic manipulations, teratological drug exposure and novelty seeking.
Journal ArticleDOI
When is the hippocampus involved in recognition memory
TL;DR: The hippocampus plays a role in recognition memory when such memory involves remembering that a particular stimulus occurred in a particular place or when the memory contains a temporal or object recency component.