PDZ and LIM domain protein 1(PDLIM1)/CLP36 promotes breast cancer cell migration, invasion and metastasis through interaction with α-actinin
TLDR
It is shown here that CLP36 is critical for promoting breast cancer cell migration and invasion in vitro and metastasis in vivo, whereas it is dispensable for breast cell proliferation and anchorage-independent growth in vitroand tumor growth in vivo.Abstract:
Increased CLP36 expression has been found to be closely associated with breast cancer progression. However, whether and how it contributes to malignant behavior of breast cancer cells were not known. We show here that CLP36 is critical for promoting breast cancer cell migration and invasion in vitro and metastasis in vivo, whereas it is dispensable for breast cell proliferation and anchorage-independent growth in vitro and tumor growth in vivo. CLP36 interacted with both α-actinin-1 and -4 in breast cancer cells. Depletion of either α-actinin-1 or -4 inhibited breast cancer cell migration. Furthermore, mutations inhibiting the α-actinin-binding activity abolished the ability of CLP36 to promote breast cancer cell migration. Finally, depletion of CLP36 or disruption of the CLP36-α-actinin complex in breast cancer cells substantially inhibited Cdc42 activation, cell polarization and migration. Our results identify CLP36 as an important regulator of breast cancer cell migration and metastasis, and shed light on how increased CLP36 expression contributes to the progression of breast cancer.read more
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References
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Journal ArticleDOI
Human CLP36, a PDZ-domain and LIM-domain protein, binds to α-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells
Kristin Bauer,Michael A. A. Kratzer,Marcus Otte,Karin Luber de Quintana,Jörg Hagmann,Georg J. Arnold,Christoph Eckerskorn,Friedrich Lottspeich,Wolfgang Siess +8 more
TL;DR: The study shows that CLP36 associates with actin filaments and stress fibers that are formed during shape change and spreading of platelets and during migration and contraction of endothelial cells, and suggests that this association might modulate the function of alpha-actinin-1.
Journal ArticleDOI
Cdc42-Interacting Protein 4 Promotes Breast Cancer Cell Invasion and Formation of Invadopodia through Activation of N-WASp
Christina S. Pichot,Constadina Arvanitis,Sean M. Hartig,Samuel A. Jensen,John Bechill,Saad Marzouk,Jindan Yu,Jeffrey A. Frost,Seth J. Corey +8 more
TL;DR: A new role for CIP4 is presented in the promotion of migration and invasion of MDA-MB-231 breast cancer cells and the contribution of F-BAR proteins to cancer cell motility and invasion is established.
Journal ArticleDOI
The PDZ-LIM protein RIL modulates actin stress fiber turnover and enhances the association of α-actinin with F-actin
TL;DR: The expression and functions of RIL originally identified as a gene downregulated in H-ras-transformed cells are studied and results implicate the RIL PDZ-LIM protein as a regulator of actin stress fiber turnover.
Journal ArticleDOI
Actinin-4 gene amplification in ovarian cancer: a candidate oncogene associated with poor patient prognosis and tumor chemoresistance
Sohei Yamamoto,Hitoshi Tsuda,Kazufumi Honda,Kaoru Onozato,Masashi Takano,Seiichi Tamai,Issei Imoto,Johji Inazawa,Tesshi Yamada,Osamu Matsubara +9 more
TL;DR: The actinin-4 gene may be a target of the 19q amplicon, acting as a candidate oncogene, and serve as a predictor of poor outcome and tumor chemoresistance in patients with advanced-stage ovarian cancers.
Journal ArticleDOI
Tumor Microenvironment-Derived Proteins Dominate the Plasma Proteome Response During Breast Cancer Induction and Progression
Sharon J. Pitteri,Karen S. Kelly-Spratt,Kay E. Gurley,Jacob J. Kennedy,Tina Busald Buson,Alice Chin,Hong Wang,Qing Zhang,Chee Hong Wong,Lewis A. Chodosh,Peter S. Nelson,Samir M. Hanash,Christopher J. Kemp +12 more
TL;DR: A systems biology approach was employed to characterize the plasma proteome response in the inducible HER2/neu mouse model of breast cancer during tumor induction, progression, and regression, offering an integrated view of tumor development relevant to plasma-based strategies to detect and diagnose cancer.