RVBs are required for assembling a functional TIP60 complex.
TLDR
RuvBL1/RVB2 proteins act as molecular adaptors that can substitute for one another to facilitate the optimal assembly, heat stability, and function of the TIP60 complex.Abstract:
RVB1/RVB2 (RuvBL1/RuvBL2 or pontin/reptin) are enigmatic AAA+ ATPase proteins that are present in multiple cellular complexes. Although they have been implicated in many cellular functions, the exact molecular function of RVB proteins in the various complexes is not clear. TIP60 complex (TIP60.com) is a tumor suppressor chromatin-remodeling complex containing RVB proteins. RVBs are required for the lysine acetyltransferase activity of TIP60.com but not for that of the pure recombinant TIP60 polypeptide. Here we describe two molecular functions of RVBs in TIP60.com. First, RVBs negate the repression of catalytic activity of TIP60 by another protein in TIP60.com, p400. RVBs competitively displace the SNF2 domain of p400 from the TIP60 polypeptide. In addition RVBs are also required for heat stability of TIP60.com by a p400-independent pathway. RVB1 and RVB2 are redundant with each other for these functions and do not require their ATPase activities. Thus, RVB proteins act as molecular adaptors that can substitute for one another to facilitate the optimal assembly, heat stability, and function of the TIP60 complex.read more
Citations
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References
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ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex
TL;DR: It is found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with hist one H2AZ in nucleosome arrays.
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TL;DR: Results indicate that chromatin remodelling driven by the Ino80 ATPase may be connected to transcription as well as DNA damage repair.
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A role for the Tip60 histone acetyltransferase in the acetylation and activation of ATM
TL;DR: It is demonstrated that DNA damage induces the rapid acetylation of ATM and the Tip60 HAT plays a key role in the activation of ATM's kinase activity in response to DNA damage.
Journal ArticleDOI
Acetylation by Tip60 Is Required for Selective Histone Variant Exchange at DNA Lesions
Thomas Kusch,Laurence Florens,W. Hayes MacDonald,Selene K. Swanson,Robert L. Glaser,John R. Yates,Susan M. Abmayr,Michael P. Washburn,Jerry L. Workman +8 more
TL;DR: It is demonstrated that the Drosophila Tip60 (dTip60) chromatin-remodeling complex acetylates nucleosomal phospho-H2Av and exchanges it with an unmodified H2Av, revealing a previously unknown mechanism for selective histone exchange that uses the concerted action of two distinct Chromatin-Remodeling enzymes within the same multiprotein complex.