Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
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Inflow water is a major source of trout farming contamination with Salmonella and multidrug resistant bacteria.
TL;DR: The contribution of Portuguese land-based intensive rainbow trout farms and retailed market trout to the spread of Salmonella and bacteria carrying clinically-relevant antibiotic resistance genes (ARGs) as well as inflow water and feed as possible sources of those contaminants is assessed.
Journal ArticleDOI
Fitness of Escherichia coli mutants with reduced susceptibility to tigecycline
TL;DR: Reduced susceptibility to tigecycline caused a decrease in fitness under stressful in vitro and in vivo conditions with ERN mutants being fitter than LPS mutants, and when combined, ERN mutations caused a synergistic increase in the MIC of tIGecy Cline.
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Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review and meta-analysis
TL;DR: In this article, the authors focused on the distribution, changes, and health risks of the bacterial antibiotic resistome (BAR) throughout the drinking water treatment system and extracted the antibiotic resistance gene (ARG) data from recent publications and analyzed ARG profiles based on diversity, absolute abundance and relative abundance.
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Antibiotic Use by Small-Scale Farmers for Freshwater Aquaculture in the Upper Mekong Delta, Vietnam.
G. Ström,Hanna Björklund,Andrew J. Barnes,Chau Thi Da,Nguyen Huu Yen Nhi,Trinh Thi Lan,Ulf Magnusson,Anna Norman Haldén,Sofia Boqvist +8 more
TL;DR: Antibiotics were used by tilapia and striped catfish farmers (84% and 69%, respectively), but not by any of the prawn farmers, which may lead to successful intervention towards reduced antibiotic use in freshwater fish farming in Vietnam.
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Monitoring of selected pharmaceuticals in surface waters of Croatia.
Martina Ivešić,Adela Krivohlavek,Irena Žuntar,Sonja Tolić,Sandra Šikić,Valerija Musić,Ivan Pavlić,Andrea Bursik,Nives Galić +8 more
TL;DR: The method was successfully applied for analysis of surface water real samples using solid-phase extraction and reversed-phase liquid chromatography-electrospray tandem mass spectrometry and the most frequent and highest concentrations were detected for macrolide antibiotics.
References
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