Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
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Journal ArticleDOI
Maximum levels of cross-contamination for 24 antimicrobial active substances in non-target feed. Part 10: Quinolones: flumequine and oxolinic acid.
Konstantinos Koutsoumanis,Ana Allende,Avelino Alvarez-Ordóñez,Declan Bolton,Sara Bover-Cid,Marianne Chemaly,Robert Davies,Alessandra De Cesare,Lieve Herman,Friederike Hilbert,Roland Lindqvist,Maarten Nauta,Giuseppe Ru,Marion Simmons,Panagiotis N. Skandamis,Elisabetta Suffredini,Dan I. Andersson,Vasileios Bampidis,Johan Bengtsson‐Palme,Damien Bouchard,Aude Ferran,Maryline Kouba,Secundino López Puente,Marta López-Alonso,Søren Saxmose Nielsen,Alena Pechová,Mariana Petkova,Sebastien Girault,Alessandro Broglia,Beatriz Guerra,Matteo Lorenzo Innocenti,Ernesto Liebana,Gloria López-Gálvez,Paola Manini,Pietro Stella,Luísa Peixe +35 more
TL;DR: The specific concentrations of flumequine and oxolinic acid in non-target feed for food-producing animals, below which there would not be an effect on the emergence of, and/or selection for, resistance in bacteria relevant for human and animal health, as well as the specific antimicrobial concentrations in feed which have an effect in terms of growth promotion/increased yield were assessed by EFSA in collaboration with EMA.
Book ChapterDOI
Antibiotic Resistance in the Aquatic Environment
TL;DR: This chapter describes the current knowledge on the emergence and spread of antibiotics resistance in the aquatic environment, with special emphasis on the role of antibiotic resistance genes.
Posted ContentDOI
Impact of sulfamethoxazole on a riverine microbiome
Chiara Borsetto,Sebastien Raguideau,Emma Rachel Travis,Dae-Wi Kim,Do-Hoon Lee,Andrew R. Bottrill,Richard Stark,Lijiang Song,Chang-Jun Cha,Jonathan M. Pearson,Christopher Quince,Andrew C. Singer,Elizabeth M. H. Wellington +12 more
TL;DR: In this article, the authors investigated the impact of sulfonamide compound sulfamethoxazole (SMX) exposure on the river bacterial microbiome using a flume system, which was a semi-natural in vitro flume using river water (30 L) and sediment with circulation to mimic river flow.
Journal ArticleDOI
The highly dynamic nature of bacterial heteroresistance impairs its clinical detection
TL;DR: In this article, the authors studied the dynamics by which these resistant subpopulations are enriched in the presence of antibiotic and recede back to their baseline frequency in the absence of selection, and they demonstrated that this test strongly underestimates the occurrence of heteroresistance in clinical isolates.
Journal ArticleDOI
Leveraging Marine Natural Products as a Platform to Tackle Bacterial Resistance and Persistence.
TL;DR: In this article, the authors discuss the dangers of antimicrobial resistance to existing antibiotics and the need to take preventive measures to further complicate the treatment of bacterial infections in the hospital setting.
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Journal ArticleDOI
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