Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
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Tools and techniques for rational designing of antimicrobial peptides for aquaculture.
Raja Aadil Hussain Bhat,Dimpal Thakuria,Ritesh Shantilal Tandel,Victoria Chanu Khangembam,Pragyan Dash,Gayatri Tripathi,Debajit Sarma +6 more
TL;DR: In this article , the authors provided a comprehensive outline about the strategies for designing synthetic mimics of natural AMPs with high potency and provided in silico template for rationally designing the AMPs from fish piscidins or other peptides.
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Concentrations of Ciprofloxacin in the World’s Rivers Are Associated with the Prevalence of Fluoroquinolone Resistance in Escherichia coli: A Global Ecological Analysis
TL;DR: Steps to reducing the concentrations of fluoroquinolones in rivers may help prevent the emergence of resistance in E. coli and other bacterial species.
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Effect of metaphylactic administration of tildipirosin on the incidence of pneumonia and otitis and on the upper respiratory tract and fecal microbiome of preweaning Holstein calves
Leonardo Bringhenti,Mariana Pallu,Josiane do Carmo Silva,Tiago Tomazi,A.C.C.H. Tomazi,Marjory Xavier Rodrigues,Livia M. Duarte,T.R. Bilby,Rodrigo C. Bicalho +8 more
TL;DR: Zuprevo et al. as discussed by the authors evaluated the effect of metaphylactic use of a semi-synthetic long-acting macrolide (tildipirosin) on the prevention of pneumonia and otitis in preweaning Holstein calves, as well as its effects on the microbiome of their upper respiratory tract and feces.
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Tailoring the immobilization and release of chlorhexidine using dopamine chemistry to fight infections associated to orthopedic devices
TL;DR: Overall data underline that one-step immobilization of CHX holds great potential to be further applied in the fight against orthopedic devices associated infections and in the biocompatibility of mammalian cells.
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Framework for establishing regulatory guidelines to control antibiotic resistance in treated effluents
TL;DR: The definition of minimal requirements and integrated monitoring are essential to map antibiotic resistance at time and space scales, and to design and implement corrective measures as discussed by the authors , which are technically and economically feasible and should be incorporated into wastewater quality directives.
References
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Journal ArticleDOI
Stochastic Gene Expression in a Single Cell
TL;DR: This work constructed strains of Escherichia coli that enable detection of noise and discrimination between the two mechanisms by which it is generated and reveals how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation.
Journal ArticleDOI
Antibiotics in the aquatic environment - A review - Part II
TL;DR: This review brings up important questions that are still open, and addresses some significant issues which must be tackled in the future for a better understanding of the behavior of antibiotics in the environment, as well as the risks associated with their occurrence.
Journal ArticleDOI
Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment
TL;DR: Global efforts are needed to promote more judicious use of prophylactic antibiotics in aquaculture as accumulating evidence indicates that unrestricted use is detrimental to fish, terrestrial animals, and human health and the environment.
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Antibiotic resistance and its cost: is it possible to reverse resistance?
Dan I. Andersson,Diarmaid Hughes +1 more
TL;DR: The findings suggest that the fitness costs of resistance will allow susceptible bacteria to outcompete resistant bacteria if the selective pressure from antibiotics is reduced, and that the rate of reversibility will be slow at the community level.
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Antibiotics and antibiotic resistance genes in natural environments.
TL;DR: The large majority of antibiotics currently used for treating infections and the antibiotic resistance genes acquired by human pathogens each have an environmental origin and the function of these elements in their environmental reservoirs may be very distinct from the “weapon-shield” role they play in clinical settings.