Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
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Adaptive resistance to antibiotics in bacteria: a systems biology perspective
TL;DR: It is proposed that one of the main cornerstones of AdR in bacteria, is the conjunction of two types of mechanisms: one rapidly responding to transient environmental challenges but not very efficient, and another much more effective and specific, but developing on longer time scales.
Journal ArticleDOI
Death and population dynamics affect mutation rate estimates and evolvability under stress in bacteria
TL;DR: It is concluded that antibiotic-induced mutagenesis is overestimated because of death and that understanding evolvability under stress requires accounting for the effects of stress on population dynamics as much as on mutation rate.
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Antibiotic Transport in Resistant Bacteria: Synchrotron UV Fluorescence Microscopy to Determine Antibiotic Accumulation with Single Cell Resolution
TL;DR: To the first time that the intracellular accumulation of a clinical antibitiotic could be determined and discussed in relation with the level of drug susceptibility for a multiresistant strain, this method to non-invasively monitor the quinolones uptake in single bacteria is developed.
Journal ArticleDOI
Antimicrobial resistance: more than 70 years of war between humans and bacteria.
Syeda Fatima Nadeem,Umar Farooq Gohar,Syed Fahad Tahir,Hamid Mukhtar,Soisuda Pornpukdeewattana,Pikunthong Nukthamna,Pikunthong Nukthamna,Ali Muhammed Moula Ali,Sri Charan Bindu Bavisetty,Salvatore Massa,Salvatore Massa +10 more
TL;DR: Tackling antimicrobial resistance requires a wide range of strategies, for example, more research in antibiotic production, the need of educating patients and the general public, as well as developing alternatives to antibiotics.
Journal ArticleDOI
High-throughput analysis of the impact of antibiotics on the human intestinal microbiota composition
S.E. Ladirat,Henk A. Schols,Arjen Nauta,Margriet H. C. Schoterman,Bart J. F. Keijser,Roy Christiaan Montijn,Harry Gruppen,Frank H. J. Schuren +7 more
TL;DR: The combination of the in-vitro fermentation screening platform with the phylogenetic microarray read-outs has shown to be reliable to simultaneously analyze the effects of several antibiotics on intestinal microbiota.
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