Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
More filters
Journal ArticleDOI
Spatial structure increases the benefits of antibiotic production in Streptomyces.
TL;DR: The results show that streptomycin is both an offensive and defensive weapon that facilitates invasion into occupied habitats and also protects against invasion by competitors, and indicate that the benefits of antibiotic production rely on ecological interactions occurring at small local scales.
Journal ArticleDOI
A new extraction procedure to abate the burden of non-extractable antibiotic residues in manure.
Larissa J. M. Jansen,Milou G.M. van de Schans,Diana de Boer,Irma E.A. Bongers,Heike Schmitt,P. Hoeksma,Bjorn J.A. Berendsen +6 more
TL;DR: The current in-house multi-residue LC-MS/MS method for manure analysis, containing 48 antibiotics, was revised, additionally validated and applied to 34 incurred manure samples.
Journal ArticleDOI
Effects of erythromycin, trimethoprim and clindamycin on attached microbial communities from an effluent dominated prairie stream
TL;DR: Because ER is so ubiquitous in receiving water bodies worldwide, the Wascana study results suggest the possibility of ecosystem disturbance elsewhere.
Journal ArticleDOI
Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study
Magdalena Pazda,Magda Rybicka,Stefan Stolte,Krzysztof P. Bielawski,Piotr Stepnowski,Jolanta Kumirska,Daniel Wolecki,Ewa Mulkiewicz +7 more
TL;DR: The main aim of the present study was to compare the occurrence of selected tetracycline and sulfonamide resistance genes in raw influent and final effluent samples from two WWTPs different in terms of size and applied biological wastewater treatment processes.
Posted ContentDOI
Bacteria primed by antimicrobial peptides develop tolerance and persist
TL;DR: It is shown that Escherichia coli primed by sublethal doses of AMPs develop tolerance and increase persistence by producing curli or colanic acid, and a population dynamic model is developed that predicts that priming delays the clearance of infections and fuels the evolution of resistance.
References
More filters
Journal ArticleDOI
Stochastic Gene Expression in a Single Cell
TL;DR: This work constructed strains of Escherichia coli that enable detection of noise and discrimination between the two mechanisms by which it is generated and reveals how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation.
Journal ArticleDOI
Antibiotics in the aquatic environment - A review - Part II
TL;DR: This review brings up important questions that are still open, and addresses some significant issues which must be tackled in the future for a better understanding of the behavior of antibiotics in the environment, as well as the risks associated with their occurrence.
Journal ArticleDOI
Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment
TL;DR: Global efforts are needed to promote more judicious use of prophylactic antibiotics in aquaculture as accumulating evidence indicates that unrestricted use is detrimental to fish, terrestrial animals, and human health and the environment.
Journal ArticleDOI
Antibiotic resistance and its cost: is it possible to reverse resistance?
Dan I. Andersson,Diarmaid Hughes +1 more
TL;DR: The findings suggest that the fitness costs of resistance will allow susceptible bacteria to outcompete resistant bacteria if the selective pressure from antibiotics is reduced, and that the rate of reversibility will be slow at the community level.
Journal ArticleDOI
Antibiotics and antibiotic resistance genes in natural environments.
TL;DR: The large majority of antibiotics currently used for treating infections and the antibiotic resistance genes acquired by human pathogens each have an environmental origin and the function of these elements in their environmental reservoirs may be very distinct from the “weapon-shield” role they play in clinical settings.