Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
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Drug-target binding quantitatively predicts optimal antibiotic dose levels in quinolones
Fabrizio Clarelli,Adam C. Palmer,Bhupender Singh,Merete Storflor,Silje Lauksund,Ted Cohen,Sören Abel,Pia Abel zur Wiesch +7 more
TL;DR: It is demonstrated that drug-target binding is a major predictor of bacterial responses to antibiotics, and COMBAT provides a framework to inform optimal antibiotic dose levels that maximize efficacy and minimize the rise of resistant mutants.
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Long-term administration of oral macrolides for acne treatment increases macrolide-resistant Propionibacterium acnes.
TL;DR: The possibility that long‐term use of oral macrolides for acne treatment facilitate the increase of macrolide‐resistant P. acnes is indicated, as well as the possibility that pre‐incubated with clindamycin improved the isolation rate of resistant mutants.
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Selective and Efficient Photoinactivation of Intracellular Staphylococcus aureus and MRSA with Little Accumulation of Drug Resistance: Application of a Ru(II) Complex with Photolabile Ligands.
Weize Sun,Yao Jian,Mengxue Zhou,Yi-Shan Yao,Na Tian,Chao Li,Jun Chen,Xuesong Wang,Qianxiong Zhou +8 more
TL;DR: In this paper, three Ru(II) complexes with photolabile ligands were explored to realize the goal of sterilizing intracellular Staphylococcus aureus.
Posted ContentDOI
Spontaneous emergence of multicellular heritability
Seyed Alireza Zamani Dahaj,Anthony Burnetti,Thomas Day,Peter Yunker,William C. Ratcliff,Matthew D. Herron +5 more
TL;DR: In this paper, the authors explored the emergence of trait heritability during the evolution of multicellularity and showed that no additional layer of genetic regulation is necessary for traits of nascent multi-cell organisms to become heritable; rather, heritability and the capacity to respond to natural selection on multicell-level traits can arise "for free".
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A novel Tn3-like composite transposon harboring blaVIM-1 in Klebsiella pneumoniae spp. pneumoniae isolated from river water.
TL;DR: A new plasmid with a novel Tn3-like transposon harboring blaVIM-1 from a Klebsiella pneumoniae strain isolated from river water in Switzerland is presented and resistance genes conferring resistance to carbapenems and other β-lactam antibiotics are inserted.
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