Selection of resistant bacteria at very low antibiotic concentrations.
Erik Gullberg,Sha Cao,Otto G. Berg,Carolina Ilbäck,Linus Sandegren,Diarmaid Hughes,Dan I. Andersson +6 more
TLDR
It is suggested that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.Abstract:
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations.read more
Citations
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Residues of 2-hydroxy-3-phenylpyrazine, a degradation product of some β-lactam antibiotics, in environmental water in Vietnam.
Nguyen Van Sy,Nguyen Van Sy,Kazuo Harada,Megumi Asayama,Minae Warisaya,Le Hong Dung,Yoshinori Sumimura,Khong Thi Diep,Le Viet Ha,Nguyen Nam Thang,Tran Thi Tuyet Hoa,Tran Minh Phu,Pham Ngoc Khai,Nguyen Thanh Phuong,Le Danh Tuyen,Yoshimasa Yamamoto,Kazumasa Hirata +16 more
TL;DR: The findings of this study suggest that HPP is a promising marker for assessing the discharge of ABPC and β-lactam antibiotics with an ABPC substructure into the environment around sampling sites.
Journal ArticleDOI
High-throughput characterization of antibiotic resistome in soil amended with commercial organic fertilizers
TL;DR: COFs are vast ARG reservoirs that should be categorized as ARG contamination sources in agriculturally related environments and increased the diversity, abundance, and potential horizontal transfer of ARGs in soil.
Journal ArticleDOI
The social network: Impact of host and microbial interactions on bacterial antibiotic tolerance and persistence
Maria C. Rocha-Granados,Blesing Zenick,Hanna E. Englander,Hanna E. Englander,Wendy W. K. Mok +4 more
TL;DR: The impact of host factors and microbial interactions on antibiotic tolerance and persistence is focused on and current efforts in leveraging the knowledge of host-microbe and microbe- microbe interactions in designing therapies that potentiate antibiotic activity and reduce the burden caused by recurrent infections are outlined.
Journal ArticleDOI
Modelling antibiotics transport in a waste stabilization pond system in Tanzania
Cathrine Christmas Møller,Johan Juhl Weisser,Sijaona C. Msigala,Robinson H. Mdegela,Sven Erik Jørgensen,Bjarne Styrishave +5 more
TL;DR: A simple dynamic model, that describes the most important removal processes of antibiotic from the wastewater stabilization pond system (WSP) “Mafisa” in Morogoro, Tanzania, was developed using STELLA® software package based on liquid chromatography tandem mass spectrometry analysis of trimethoprim.
Journal ArticleDOI
Long-term effects of cataract surgery with topical levofloxacin on ocular bacterial flora
Takashi Ono,R. Nejima,Takuya Iwasaki,Yosai Mori,Yukari Noguchi,Akiko Yagi,Hideaki Hanaki,Kazunori Miyata +7 more
TL;DR: The restoration of the bacterial flora required more than 6 months after cataract surgery and topical levofloxacin, and Staphylococcus epidermidis isolates and Propionibacterium acnes did not show significant changes in the geometric mean lev oflOxacin MIC over time.
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