Severe reduction in leukocyte adhesion and monocyte extravasation in mice deficient in CC chemokine receptor 2
William A. Kuziel,Sharon J. Morgan,Tracey C. Dawson,Stephanie Griffin,Oliver Smithies,Klaus Ley,Nobuyo Maeda +6 more
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Results demonstrate that CCR2 is a major regulator of induced macrophage trafficking in vivo and shows enhanced early accumulation and delayed clearance of neutrophils and eosinophils.Abstract:
CC chemokine receptor 2 (CCR2) is a prominent receptor for the monocyte chemoattractant protein (MCP) group of CC chemokines. Mice generated by gene targeting to lack CCR2 exhibit normal leukocyte rolling but have a pronounced defect in MCP-1-induced leukocyte firm adhesion to microvascular endothelium and reduced leukocyte extravasation. Constitutive macrophage trafficking into the peritoneal cavity was not significantly different between CCR2-deficient and wild-type mice. However, after intraperitoneal thioglycollate injection, the number of peritoneal macrophages in CCR2-deficient mice did not rise above basal levels, whereas in wild-type mice the number of macrophages at 36 h was ≈3.5 times the basal level. The CCR2-deficient mice showed enhanced early accumulation and delayed clearance of neutrophils and eosinophils. However, by 5 days neutrophils and eosinophils in both CCR2-deficient and wild-type mice had returned to near basal levels, indicating that resolution of this inflammatory response can occur in the absence of macrophage influx and CCR2-mediated activation of the resident peritoneal macrophages. After intravenous injection with yeast β-glucan, wild-type mice formed numerous large, well-defined granulomas throughout the liver parenchyma, whereas CCR2-deficient mice had much fewer and smaller granulomas. These results demonstrate that CCR2 is a major regulator of induced macrophage trafficking in vivo.read more
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Monocyte and macrophage heterogeneity
Siamon Gordon,Philip R. Taylor +1 more
TL;DR: Recent studies have shown that monocyte heterogeneity is conserved in humans and mice, allowing dissection of its functional relevance: the different monocyte subsets seem to reflect developmental stages with distinct physiological roles, such as recruitment to inflammatory lesions or entry to normal tissues.
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Monocyte recruitment during infection and inflammation
Chao Shi,Eric G. Pamer +1 more
TL;DR: The mechanisms that control monocyte trafficking under homeostatic, infectious and inflammatory conditions are being unravelled and are the focus of this Review.
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International Union of Pharmacology. XXII. Nomenclature for Chemokine Receptors
Philip M. Murphy,Marco Baggiolini,Israel F. Charo,Caroline A. Hébert,Richard Horuk,Kouji Matsushima,Louis H. Miller,Joost J. Oppenheim,Christine A. Power +8 more
TL;DR: A widely accepted receptor nomenclature system is described, ratified by the International Union of Pharmacology, that is facilitating clear communication in this area and updating current concepts of the biology and pharmacology of the chemokine system.
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Tissue-Resident Macrophages Self-Maintain Locally throughout Adult Life with Minimal Contribution from Circulating Monocytes
Daigo Hashimoto,Andrew Chow,Andrew Chow,Clara Noizat,Clara Noizat,Pearline Teo,Mary Beth Beasley,Marylene Leboeuf,Christian Becker,Peter See,Jeremy Price,Daniel Lucas,Melanie Greter,Melanie Greter,Arthur Mortha,Scott W. Boyer,E. Camilla Forsberg,Masato Tanaka,Nico van Rooijen,Adolfo García-Sastre,E. Richard Stanley,Florent Ginhoux,Paul S. Frenette,Miriam Merad +23 more
TL;DR: Results indicate that tissue-resident macrophages and circulating monocytes should be classified as mononuclear phagocyte lineages that are independently maintained in the steady state.
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Absence of Monocyte Chemoattractant Protein-1 Reduces Atherosclerosis in Low Density Lipoprotein Receptor–Deficient Mice
Long Gu,Yoshikatsu Okada,Steven K. Clinton,Craig Gerard,Galina K. Sukhova,Peter Libby,Barrett J. Rollins +6 more
TL;DR: Monocyte chemoattractant protein-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.
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